L-Selectin anticorps

N° du produit ABIN2749031

Détail du produit anti-L-Selectin anticorps

Antigène: L-Selectin (SELL) (Selectin L (SELL))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp L-Selectin est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificité: The mouse monoclonal antibody DREG56 recognizes an extracellular epitope of CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: PMA-activated human peripheral blood leukocytes

Clone: DREG56

Isotype: IgG1

Information d'application

Indications d'application: Functional application: Particularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymph node HEV.
Flow cytometry: Recommended dilution: 2 μg/mL.
Western blotting: Non-reducing conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-L-Selectin (ABIN2749031)

Tu, Mao, Zheng, Liu, Chiu, Qin, Chan, Lam, Guan, Zhang, Guan, Yuen, Peiris, Lau: "Cytotoxic T lymphocytes established by seasonal human influenza cross-react against 2009 pandemic H1N1 influenza virus." dans: Journal of virology, Vol. 84, Issue 13, pp. 6527-35, (2010) (PubMed).

Killock, Parsons, Zarrouk, Ameer-Beg, Ridley, Haskard, Zvelebil, Ivetic: "In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin." dans: The Journal of biological chemistry, Vol. 284, Issue 13, pp. 8833-45, (2009) (PubMed).

Xu, Chen, Shang, Cui, Luo, Chen, Ba, Zeng: "Critical role of Lck in L-selectin signaling induced by sulfatides engagement." dans: Journal of leukocyte biology, Vol. 84, Issue 4, pp. 1192-201, (2008) (PubMed).

Jutila, Kurk, Jackiw, Knibbs, Stoolman: "L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 4, pp. 1768-73, (2002) (PubMed).

Kishimoto, Warnock, Jutila, Butcher, Lane, Anderson, Smith: "Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro." dans: Blood, Vol. 78, Issue 3, pp. 805-11, (1991) (PubMed).

Kishimoto, Jutila, Butcher: "Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 6, pp. 2244-8, (1990) (PubMed).

Détails sur L-Selectin

Antigène: L-Selectin (SELL) (Selectin L (SELL))

Autre désignation: CD62L (SELL Produits)

Synonymes: anticorps CD62L, anticorps LAM1, anticorps LECAM1, anticorps LEU8, anticorps LNHR, anticorps LSEL, anticorps LYAM1, anticorps PLNHR, anticorps TQ1, anticorps A.11, anticorps L-selectin, anticorps LECAM-1, anticorps SELE, anticorps AI528707, anticorps Lnhr, anticorps Ly-22, anticorps Ly-m22, anticorps Lyam-1, anticorps Lyam1, anticorps selectin L, anticorps selectin, lymphocyte, anticorps SELL, anticorps Sell

Sujet: Selectin L,CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).,L-selectin, LSEL, SELL, LAM-1, LECAM1, LEU8, LNHR, LYAM1, PLNHR

ID gène: 6402

UniProt: P14151