P-Selectin anticorps

N° du produit ABIN2749032

Il existe 2+ publications pour ce produit. L’anticorps anti-P-Selectin Monoclonal Souris est utilisé pour la détection de P-Selectin dans des échantillons de Humain et Non-Human Primate. Il a été validé pour WB, FACS, IP, ICC, IHC (fro) et Func.

Aperçu rapide pour P-Selectin anticorps (ABIN2749032)

Antigène: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp P-Selectin est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: AK4

Détail du produit anti-P-Selectin anticorps

Fonction: Anti-Hu CD62P Purified Low Endotoxin

Réactivité croisée (Details): Human, Non-Human Primates

Attributs du produit: The antibody AK4 recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes, it is relocated to the plasma membrane upon activation.

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: Human platelets

Isotype: IgG1, kappa

Information d'application

Indications d'application: Functional application: Blocking.
Flow cytometry: Recommended dilution: 1.5 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Conseil sur la manipulation: Do not freeze

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-P-Selectin (ABIN2749032)

Kowalska, Ratajczak, Hoxie, Brass, Gewirtz, Poncz, Ratajczak: "Megakaryocyte precursors, megakaryocytes and platelets express the HIV co-receptor CXCR4 on their surface: determination of response to stromal-derived factor-1 by megakaryocytes and platelets." dans: British journal of haematology, Vol. 104, Issue 2, pp. 220-9, (1999) (PubMed).

Dunlop, Skinner, Bendall, Favaloro, Castaldi, Gorman, Gamble, Vadas, Berndt: "Characterization of GMP-140 (P-selectin) as a circulating plasma protein." dans: The Journal of experimental medicine, Vol. 175, Issue 4, pp. 1147-50, (1992) (PubMed).

Détails sur P-Selectin

Antigène: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Autre désignation: CD62P

Sujet: Selectin P,CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.,P-selectin, GMP140, SELP, PADGEM, GRMP, LECAM3, PSEL

ID gène: 6403

UniProt: P16109