Transferrin anticorps

N° du produit ABIN2749040

Détail du produit anti-Transferrin anticorps

Antigène: Transferrin (TF)

Reactivité: Humain, Porc, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transferrin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Radioimmunoassay (RIA), Functional Studies (Func)

Specificité: The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.

Aucune reactivité croisée: Boeuf (Vache), Chien, Cheval, Mouton

Réactivité croisée (Details): Human, Rabbit, Porcine

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: Purified porcine transferrin.

Clone: HTF-14

Isotype: IgG1

Information d'application

Indications d'application: Functional application: The antibody HTF-14 blocks binding of transferrin to its receptor.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: placenta.
Western blotting: non-reducing conditions, recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-Transferrin (ABIN2749040)

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." dans: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, (1996) (PubMed).

Bártek, Viklický, Stratil: "Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding." dans: British journal of haematology, Vol. 59, Issue 3, pp. 435-41, (1985) (PubMed).

Bártek, Viklický, Fran?k, Angelisová, Dráber, Jarosíková, N?mec, Verlová: "Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity." dans: Immunology letters, Vol. 4, Issue 5, pp. 231-5, (1982) (PubMed).

Détails sur Transferrin

Antigène: Transferrin (TF)

Autre désignation: Transferrin (TF Produits)

Synonymes: anticorps ltf, anticorps pro1557, anticorps pro2086, anticorps mgc107777, anticorps LOC692564, anticorps TF, anticorps LOC100144362, anticorps tf, anticorps LTF, anticorps TFEW, anticorps conalbumin, anticorps tf-b, anticorps MGC64306, anticorps PRO1557, anticorps PRO2086, anticorps TFQTL1, anticorps AI266983, anticorps Cd176, anticorps HP, anticorps Tf, anticorps Tfn, anticorps hpx, anticorps Trf, anticorps cb285, anticorps gavi, anticorps id:ibd3238, anticorps id:ibd3525, anticorps sb:cb285, anticorps wu:fb57g06, anticorps wu:fb62h02, anticorps wu:fb63h10, anticorps wu:fb64h10, anticorps zgc:112154, anticorps 143958_at, anticorps CG6186, anticorps Dmel\\CG6186, anticorps TSF1, anticorps anon-EST:Posey265, anticorps tsf1, anticorps Pro-TRH, anticorps IL-5, anticorps STF I, anticorps TRF1, anticorps sTF1, anticorps sTf, anticorps tf1, anticorps transferrin, anticorps serotransferrin, anticorps transferrin (ovotransferrin), anticorps transferrin L homeolog, anticorps melanotransferrin, anticorps transferrin-a, anticorps Transferrin 1, anticorps thyrotropin releasing hormone, anticorps interleukin 5, anticorps TF, anticorps LOC477072, anticorps tf, anticorps Tf, anticorps LOC100144362, anticorps tf.L, anticorps LOC5575625, anticorps Trf, anticorps tfa, anticorps Tsf1, anticorps TRH, anticorps IL5, anticorps LOC101085148, anticorps LOC100726872, anticorps trf

Sujet: Transferrin,Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.,TFQTL1, PRO1557, PRO2086

ID gène: 7018

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis