MICA anticorps

N° du produit ABIN2749085

Détail du produit anti-MICA anticorps

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Reactivité: Humain, Boeuf (Vache), Cat, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MICA est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytometry by Time of Flight (CyTOF)

Specificité: The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.

Aucune reactivité croisée: Lapin

Réactivité croisée (Details): Human, Non-Human Primates, Bovine, Feline (Cat)

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Membrane of human tonsil cells

Clone: W6-32

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-12 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-MICA (ABIN2749085)

Le Discorde, Moreau, Sabatier, Legeais, Carosella: "Expression of HLA-G in human cornea, an immune-privileged tissue." dans: Human immunology, Vol. 64, Issue 11, pp. 1039-44, (2003) (PubMed).

Tran, Ivanyi, Hilgert, Brdicka, Pla, Breur, Flieger, Ivasková, Horejsí: "The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex." dans: Immunogenetics, Vol. 53, Issue 6, pp. 440-6, (2001) (PubMed).

Ladasky, Shum, Canavez, Seuánez, Parham: "Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody." dans: Immunogenetics, Vol. 49, Issue 4, pp. 312-20, (1999) (PubMed).

Shields, Ribaudo: "Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin." dans: Tissue antigens, Vol. 51, Issue 5, pp. 567-70, (1998) (PubMed).

Jacobsen, Aasted, Broe, Petersen: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." dans: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, (1994) (PubMed).

Stern, Beresford, Friedman, Stevens, Risk, Johnson: "Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 4, pp. 1088-91, (1987) (PubMed).

Neefjes, Hensen, de Kroon, Ploegh: "A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes." dans: Immunogenetics, Vol. 23, Issue 5, pp. 341-7, (1986) (PubMed).

Brodsky, Parham: "Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies." dans: Immunogenetics, Vol. 15, Issue 2, pp. 151-66, (1982) (PubMed).

Barnstable, Bodmer, Brown, Galfre, Milstein, Williams, Ziegler: "Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis." dans: Cell, Vol. 14, Issue 1, pp. 9-20, (1978) (PubMed).

Détails sur MICA

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Autre désignation: HLA-Class I (MICA Produits)

Synonymes: anticorps MIC-A, anticorps PERB11.1, anticorps MHC class I polypeptide-related sequence A, anticorps MICA

Sujet: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response