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kappa Light Chain anticorps (Biotin)

Reactivité: Humain FACS Hôte: Souris Monoclonal TB28-2 Biotin
N° du produit ABIN2749092
  • Antigène Voir toutes kappa Light Chain Anticorps
    kappa Light Chain
    Reactivité
    • 33
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    • 1
    Humain
    Hôte
    • 24
    • 23
    • 2
    • 1
    • 1
    Souris
    Clonalité
    • 26
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    Monoclonal
    Conjugué
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
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    Cet anticorp kappa Light Chain est conjugé à/à la Biotin
    Application
    • 31
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    Flow Cytometry (FACS)
    Specificité
    The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.
    Réactivité croisée (Details)
    Human
    Purification
    Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
    Immunogène
    Human IgG-kappa myeloma protein
    Clone
    TB28-2
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product kappa Light Chain Anticorps primaire
  • Indications d'application
    Flow cytometry: Recommended dilution: 1-12 μg/mL. Extracellular and intracellular staining.
    Commentaires

    The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Jourdan, Caraux, De Vos, Fiol, Larroque, Cognot, Bret, Duperray, Hose, Klein: "An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization." dans: Blood, Vol. 114, Issue 25, pp. 5173-81, (2009) (PubMed).

    Böttcher, Ritgen, Buske, Gesk, Klapper, Hoster, Hiddemann, Unterhalt, Dreyling, Siebert, Kneba, Pott et al.: "Minimal residual disease detection in mantle cell lymphoma: methods and significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for ..." dans: Haematologica, Vol. 93, Issue 4, pp. 551-9, (2008) (PubMed).

    Karandikar, Aquino, McKenna, Kroft: "Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome. An immunophenotypic analysis." dans: American journal of clinical pathology, Vol. 116, Issue 2, pp. 204-10, (2001) (PubMed).

    Nakamura, Kubagawa, Cooper: "Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8522-6, (1992) (PubMed).

    Kiyotaki, Cooper, Bertoli, Kearney, Kubagawa: "Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4150-8, (1987) (PubMed).

  • Antigène
    kappa Light Chain
    Autre désignation
    Kappa light chains (kappa Light Chain Produits)
    Synonymes
    anticorps LOC100037688, anticorps B128L, anticorps B55L, anticorps IgK, anticorps TCRAV31S1, anticorps TCRAV40S1, anticorps kappa light chain, anticorps immunoglobulin kappa chain variable 8 (V8), anticorps T cell receptor alpha variable 40, anticorps LOC100037688, anticorps Igk-V8, anticorps TRAV40
    Sujet
    Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.,Immunoglobulin kappa, Igk
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