CD40 Ligand anticorps (PE)

N° du produit ABIN2749175

Détail du produit anti-CD40 Ligand anticorps (PE)

Antigène: CD40 Ligand (CD40LG)

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD40 Ligand est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Specificité: The mouse monoclonal antibody 24-31 detects an extracellular epitope of CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.

Réactivité croisée (Details): Human, Non-Human Primates

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: human CD154 fusion protein

Clone: 24-31

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD40 Ligand (PE) (ABIN2749175)

Fernandes, Gomes, Butcher, Hernandez-Alcoceba, Chang, Kansopon, Newman, Stone, Tong: "Growth inhibition of human multiple myeloma cells by an oncolytic adenovirus carrying the CD40 ligand transgene." dans: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 15, pp. 4847-56, (2009) (PubMed).

Kum, Hung, Cameron, Chow et al.: "Temporal sequence and functional implications of V beta-specific T cell receptor down-regulation and costimulatory molecule expression following in vitro stimulation with the staphylococcal ..." dans: The Journal of infectious diseases, Vol. 185, Issue 4, pp. 555-60, (2002) (PubMed).

Brams, Black, Padlan, Hariharan, Leonard, Chambers-Slater, Noelle, Newman: "A humanized anti-human CD154 monoclonal antibody blocks CD154-CD40 mediated human B cell activation." dans: International immunopharmacology, Vol. 1, Issue 2, pp. 277-94, (2001) (PubMed).

Barnhart, Ford, Bhushan, Song, Covey: "A polymorphic CD40 ligand (CD154) molecule mediates CD40-dependent signalling but interferes with the ability of soluble CD40 to functionally block CD154:CD40 interactions." dans: Immunology, Vol. 99, Issue 1, pp. 54-61, (2000) (PubMed).

Berner, Wolf, Hummel, Müller, Reuss-Borst: "Increased expression of CD40 ligand (CD154) on CD4+ T cells as a marker of disease activity in rheumatoid arthritis." dans: Annals of the rheumatic diseases, Vol. 59, Issue 3, pp. 190-5, (2000) (PubMed).

Grammer, McFarland, Heaney, Darnell, Lipsky: "Expression, regulation, and function of B cell-expressed CD154 in germinal centers." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 8, pp. 4150-9, (1999) (PubMed).

Kornbluth, Kee, Richman: "CD40 ligand (CD154) stimulation of macrophages to produce HIV-1-suppressive beta-chemokines." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 9, pp. 5205-10, (1998) (PubMed).

Détails sur CD40 Ligand

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD154 / CD40L (CD40LG Produits)

Synonymes: anticorps CD154, anticorps cd40l, anticorps CD40L, anticorps HIGM1, anticorps IGM, anticorps IMD3, anticorps T-BAM, anticorps TNFSF5, anticorps TRAP, anticorps gp39, anticorps hCD40L, anticorps CD40-L, anticorps Cd40l, anticorps Ly-62, anticorps Ly62, anticorps Tnfsf5, anticorps CD40 ligand, anticorps TNF superfamily member 5, anticorps CD40LG, anticorps cd40l, anticorps Cd40lg

Sujet: CD40 ligand,CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.,CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM

ID gène: 21947

UniProt: P27548

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints