CD82 anticorps

N° du produit ABIN2749189

Détail du produit anti-CD82 anticorps

Antigène: CD82

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD82 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)

Specificité: The mouse monoclonal antibody C33 recognizes an extracellular/luminal epitope of CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.

Réactivité croisée (Details): Human, Other not tested

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: C91/PL (human HTLV-1+ T cell line)

Clone: C33

Isotype: IgG2a

Information d'application

Indications d'application: Functional application: In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD82 (ABIN2749189)

Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." dans: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Escola, Kleijmeer, Stoorvogel, Griffith, Yoshie, Geuze: "Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes." dans: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20121-7, (1998) (PubMed).

Ueda, Ichikawa, Tamaru, Mikata, Akakura, Akimoto, Imai, Yoshie, Shiraishi, Yatani, Ito, Shimazaki: "Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer." dans: The American journal of pathology, Vol. 149, Issue 5, pp. 1435-40, (1996) (PubMed).

Imai, Kakizaki, Nishimura, Yoshie: "Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1229-39, (1995) (PubMed).

Imai, Yoshie et al.: "C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and ..." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 6470-81, (1994) (PubMed).

Fukudome, Furuse, Imai, Nishimura, Takagi, Hinuma, Yoshie et al.: "Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen ..." dans: Journal of virology, Vol. 66, Issue 3, pp. 1394-401, (1992) (PubMed).

Détails sur CD82

Antigène: CD82

Autre désignation: CD82 (CD82 Produits)

Synonymes: anticorps AA682076, anticorps AL023070, anticorps C33, anticorps IA4, anticorps Kai1, anticorps Tspan27, anticorps 4F9, anticorps GR15, anticorps KAI1, anticorps R2, anticorps SAR2, anticorps ST6, anticorps TSPAN27, anticorps CD82 antigen, anticorps CD82 molecule, anticorps Cd82 molecule, anticorps Cd82, anticorps CD82

Sujet: CD82 Molecule,CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.,R2, 4F9, C33, IA4, ST6, GR15, KAI1, SAR2, TSPAN27

ID gène: 3732

UniProt: P27701

Pathways: Signalisation p53