CD180 anticorps

N° du produit ABIN2749219

Détail du produit anti-CD180 anticorps

Antigène: CD180 (CD180 Molecule (CD180))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD180 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Specificité: The mouse monoclonal antibody G28-8 recognizes an extracellular epitope of CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: Human tonsillar B cells

Clone: G28-8

Isotype: IgG1 kappa

Information d'application

Indications d'application: Functional application: Induction of B cell proliferation.
Flow cytometry: Recommended dilution: 2 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD180 (ABIN2749219)

Olson, Graves, Shu, Ryan, Clark: "Caspase activity is required for stimulated B lymphocytes to enter the cell cycle." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 6065-72, (2003) (PubMed).

Clark, Shu, Lüscher, Draves, Banchereau, Ledbetter, Valentine: "Activation of human B cells. Comparison of the signal transduced by IL-4 to four different competence signals." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 12, pp. 3873-80, (1990) (PubMed).

Valentine, Clark, Shu, Norris, Ledbetter: "Antibody to a novel 95-kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 12, pp. 4071-8, (1988) (PubMed).

Détails sur CD180

Antigène: CD180 (CD180 Molecule (CD180))

Autre désignation: CD180 (CD180 Produits)

Synonymes: anticorps Ly78, anticorps CD180, anticorps LY64, anticorps RP105, anticorps F630107B15, anticorps CD180 molecule, anticorps CD180 antigen, anticorps Cd180, anticorps CD180

Sujet: CD180 Molecule,CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1, and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.,LY64, Ly78, RP105, Bgp95

ID gène: 4064

UniProt: Q99467

Pathways: Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades