CD86 anticorps

N° du produit ABIN2749230

Détail du produit anti-CD86 anticorps

Antigène: CD86

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD86 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificité: The mouse monoclonal antibody BU63 reacts with an extracellular epitope of CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Réactivité croisée (Details): Human, Other not determined

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: B-lymphoblastoid cell line ARH 77

Clone: BU63

Isotype: IgG1

Information d'application

Indications d'application: Functional application: The antibody BU63 blocks mixed lymphocyte reaction (MLR) and binding of soluble CTLA-4 (CD152)-mulg fusion protein to CD86 (B7-2).
Flow cytometry: Recommended dilution: 5 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD86 (ABIN2749230)

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." dans: BMC immunology, Vol. 12, pp. 2, (2011) (PubMed).

Kolar, Mehta, Pelayo, Capra: "A novel human B cell subpopulation representing the initial germinal center population to express AID." dans: Blood, Vol. 109, Issue 6, pp. 2545-52, (2007) (PubMed).

Chan, Baird, Mercer, Fleming: "Maturation and function of human dendritic cells are inhibited by orf virus-encoded interleukin-10." dans: The Journal of general virology, Vol. 87, Issue Pt 11, pp. 3177-81, (2006) (PubMed).

Zhan, Towler, Calder: "The immunomodulatory role of human conjunctival epithelial cells." dans: Investigative ophthalmology & visual science, Vol. 44, Issue 9, pp. 3906-10, (2003) (PubMed).

Mauri, Wyss-Coray, Gallati, Pichler: "Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 118-27, (1995) (PubMed).

Caux, Vanbervliet, Massacrier, Azuma, Okumura, Lanier, Banchereau: "B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells." dans: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1841-7, (1994) (PubMed).

Engel, Gribben, Freeman, Zhou, Nozawa, Abe, Nadler, Wakasa, Tedder: "The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen." dans: Blood, Vol. 84, Issue 5, pp. 1402-7, (1994) (PubMed).

Détails sur CD86

Antigène: CD86

Autre désignation: CD86 (CD86 Produits)

Synonymes: anticorps B7-2, anticorps B7.2, anticorps B70, anticorps CD28LG2, anticorps LAB72, anticorps B7, anticorps CLS1, anticorps Cd28l2, anticorps ETC-1, anticorps Ly-58, anticorps Ly58, anticorps MB7, anticorps MB7-2, anticorps TS/A-2, anticorps CD86 molecule, anticorps CD86 antigen, anticorps integrin subunit alpha 2b, anticorps CD86, anticorps Cd86, anticorps Itga2b

Sujet: CD86 Molecule,CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.,B7-2, FUN-1, LAB72

ID gène: 942

UniProt: P42081

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation