UPF3B anticorps (N-Term)

N° du produit ABIN2776563

Détail du produit anti-UPF3B anticorps

Antigène: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Chien, Lapin, Porc, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UPF3B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Séquence: MKEEKEHRPK EKRVTLLTPA GATGSGGGTS GDSSKGEDKQ DRNKEKKEAL

Homologie: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 93%, Rat: 100%

Attributs du produit: This is a rabbit polyclonal antibody against UPF3B. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human UPF3B

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 470 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-UPF3B (ABIN2776563)

Mooney, Jimenez-Mateos, Engel, Mooney, Diviney, Venø, Kjems, Farrell, OBrien, Delanty, Henshall: "RNA sequencing of synaptic and cytoplasmic Upf1-bound transcripts supports contribution of nonsense-mediated decay to epileptogenesis." dans: Scientific reports, Vol. 7, pp. 41517, (2017) (PubMed).

Détails sur UPF3B

Antigène: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Autre désignation: UPF3B (UPF3B Produits)

Sujet: UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The protein is one of two functional homologs to yeast Upf3p. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctionsThis gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.
Alias Symbols: UPF3X, HUPF3B, MRXS14, RENT3B
Protein Interaction Partner: NCBP1, CIAO1, STAU1, UBC, UPF1, HECW2, RBM8A, UPF2, SMG1, EIF4A3, WIBG, WBP11, RNPS1, SART1, TOP2A, SNRPA1, NXF1, CAND1, COPS5, CUL5, USP21, EXOSC4, MCRS1, XRN1, TTC19, EIF6, UPF3B, HBB, UPF3A,
Protein Size: 470

Poids moléculaire: 52 kDa

ID gène: 65109

NCBI Accession: NM_023010, NP_075386

UniProt: Q9BZI7