ERI1 anticorps (N-Term)

N° du produit ABIN2776656

Détail du produit anti-ERI1 anticorps

Antigène: ERI1 (Exoribonuclease 1 (ERI1))

Épitope: N-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERI1 est non-conjugé

Application: Western Blotting (WB)

Séquence: SKFITSSASD FSDPVYKEIA ITNGCINRMS KEELRAKLSE FKLETRGVKD

Homologie: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 85%, Pig: 93%, Rabbit: 100%, Rat: 79%

Attributs du produit: This is a rabbit polyclonal antibody against THEX1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human ERI1

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 349 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-ERI1 (ABIN2776656)

Kupsco, Wu, Marzluff, Thapar, Duronio: "Genetic and biochemical characterization of Drosophila Snipper: A promiscuous member of the metazoan 3'hExo/ERI-1 family of 3' to 5' exonucleases." dans: RNA (New York, N.Y.), Vol. 12, Issue 12, pp. 2103-17, (2006) (PubMed).

Détails sur ERI1

Antigène: ERI1 (Exoribonuclease 1 (ERI1))

Autre désignation: THEX1 (ERI1 Produits)

Synonymes: anticorps THEX1, anticorps thex1, anticorps zgc:110635, anticorps hexo, anticorps Eri-1, anticorps 3'HEXO, anticorps HEXO, anticorps 3'hexo, anticorps 3110010F15Rik, anticorps Thex1, anticorps eri-1, anticorps RGD1308378, anticorps exoribonuclease 1, anticorps three prime repair exonuclease 1, anticorps exoribonuclease 1 L homeolog, anticorps 3'-5' exonuclease eri-1, anticorps ERI1, anticorps CpipJ_CPIJ003945, anticorps eri1, anticorps eri1.L, anticorps Eri1, anticorps eri-1

Sujet: RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
Alias Symbols: HEXO, THEX1, 3'HEXO
Protein Interaction Partner: UBC, HNRNPD, HHV8GK18_gp81, SLBP,
Protein Size: 38

Poids moléculaire: 349 kDa

ID gène: 90459

NCBI Accession: NM_153332, NP_699163

UniProt: Q8IV48