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beta Actin anticorps (Middle Region)

Name: beta Actin anticorps (Middle Region)
SKU: ABIN501930
Price: 150.78 EUR
Availability: InStock

ACTB Reactivité: Humain, Souris, Rat, Porc, Mouton, Boeuf (Vache), Poisson zèbre (Danio rerio), Cheval, Chévre WB, IHC Hôte: Lapin Polyclonal unconjugated

WB Suggested Anti-ACTB Antibody Positive Control: Lane 1: 341 µg mouse 3T3 ECM extract + blocking peptide, Lane 2: 041 µg mouse 3T3 ECM extract, Lane 3: 041 µg Echinococcus granulosus extract Primary Antibody Dilution: 1:000Secondary Antibody: Anti-rabbit-HRP Secondry Antibody Dilution: 1:00,000Submitted by: Anonymous (beta Actin anticorps (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 µg/mL Peptide Concentration: 5. (beta Actin anticorps (Middle Region))

Sample Type: NT2 cells Red: Antibody Blue: DAPI Primary Dilution: 1ug/50ul antibody Secondary Antibody: Alexa goat anti-rabbit 594 Image Submitted by: Yuzhi Chen, University of Arkansas for Medical Sciences (beta Actin anticorps (Middle Region))

IHC

Sample type: 1-8. (beta Actin anticorps (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Human Adult Placenta Antibody Dilution: 1. (beta Actin anticorps (Middle Region))

Host: Rabbit Target Name: ACTBM Sample Type: Fetal Lung lysates Antibody Dilution: 1ug/ml (beta Actin anticorps (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: MCF7 Antibody Dilution: 1. (beta Actin anticorps (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 1. (beta Actin anticorps (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Human Fetal Lung Antibody Dilution: 1. (beta Actin anticorps (Middle Region))

WB Suggested Anti-ACTB Antibody Titration: 0. (beta Actin anticorps (Middle Region))

Host: Mouse Target Name: ACTG1 Sample Tissue: Mouse Testis Antibody Dilution: 1ug/ml (beta Actin anticorps (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: Mouse Heart Antibody Dilution: 1ug/ml (beta Actin anticorps (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Hela Antibody Dilution: 1. (beta Actin anticorps (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: Human HepG2 Antibody Dilution: 1. (beta Actin anticorps (Middle Region))

Sample Type : Human brain stem cells Primary Antibody Dilution : 1:500 Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594 Secondary Antibody Dilution : 1:1000 Color/Signal Descriptions : ACTB: Red DAPI:Blue Gene Name : ACTB Submitted by : Dr. (beta Actin anticorps (Middle Region))

IHC

Host: Rabbit Target Name: ACTB Sample Type: HepG2 Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1ug/ml Peptide Concentration: 5. (beta Actin anticorps (Middle Region))

IHC Information: Paraffin embedded breast tissue, tested with an antibody dilution of 5 ug/ml. (beta Actin anticorps (Middle Region))

IHC

Host: Rabbit Target Name: ACTB Sample Type: Jurkat Antibody Dilution: 1. (beta Actin anticorps (Middle Region))

Rabbit Anti-ACTB Antibody Formalin Fixed Paraffin Embedded Tissue: Human Appendix (Colon) Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0. (beta Actin anticorps (Middle Region))

IHC

20 images

(2 références)

N° du produit ABIN501930

Fiche de données

Antigène Voir toutes beta Actin (ACTB) Anticorps

beta Actin (ACTB) (Actin, beta (ACTB))
Épitope
40

18

16

6

6

5

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Middle Region
Reactivité
194

138

119

65

52

44

37

36

32

25

20

20

12

10

9

8

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
Humain, Souris, Rat, Porc, Mouton, Boeuf (Vache), Poisson zèbre (Danio rerio), Cheval, Chévre
Hôte
115

95

5

1

1
Lapin
Anticorps secondaires appropriés
Clonalité
114

101
Polyclonal
Conjugué
131

14

13

11

4

4

4

4

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp beta Actin est non-conjugé
Application
176

78

60

51

35

30

27

22

13

13

6

6

6

5

5

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

Séquence

TMKIKIIAPP ERKYSVWIGG SILASLSTFQ QMWISKQEYD ESGPSIVHRK

Homologie

Cow: 100%, Goat: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Attributs du produit

This is a rabbit polyclonal antibody against ACTB. It was validated on Western Blot.

Purification

Affinity Purified

Immunogène

The immunogen is a synthetic peptide directed towards the middle region of Human ACTB

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ACTB Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache) WB, IHC (p) Hôte: Souris Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivité: Humain WB, IHC Hôte: Lapin Polyclonal unconjugated

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ACTB Reactivité: Humain WB, ELISA, IHC (p) Hôte: Souris Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB Reactivité: Humain WB, IF, IHC (p), ICC Hôte: Lapin Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivité: Humain, Souris, Poulet, Lapin, Porc, Boeuf (Vache), Hamster, Chévre, Poisson WB, ELISA Hôte: Souris Monoclonal 2D1D10 unconjugated

Indications d'application

Optimal working dilutions should be determined experimentally by the investigator.

Commentaires

Antigen size: 375 AA

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and may contain up to 2 % sucrose.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation

Avoid repeated freeze-thaw cycles.

Stock

-20 °C

Stockage commentaire

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Phungphong, Kijtawornrat, Wattanapermpool, Bupha-Intr: "Regular exercise modulates cardiac mast cell activation in ovariectomized rats." dans: The journal of physiological sciences : JPS, Vol. 66, Issue 2, pp. 165-73, (2016) (PubMed).

Rinne, Clements, Lamme, Duijf, Bolat, Meijer, Scheffer, Rosser, Tan, McGrath, Schalkwijk, Brunner, Zhou, van Bokhoven: "A novel translation re-initiation mechanism for the p63 gene revealed by amino-terminal truncating mutations in Rapp-Hodgkin/Hay-Wells-like syndromes." dans: Human molecular genetics, Vol. 17, Issue 13, pp. 1968-77, (2008) (PubMed).
Antigène

beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation

ACTB (ACTB Produits)

Synonymes

anticorps BRWS1, anticorps PS1TP5BP1, anticorps ACTA1, anticorps Actx, anticorps E430023M04Rik, anticorps beta-actin, anticorps ps1tp5bp1, anticorps act-2, anticorps actb, anticorps B-actin, anticorps LOC100174883, anticorps LOC100304816, anticorps A, anticorps ACT5C, anticorps Ac5C, anticorps Act, anticorps Act-5C, anticorps Act5, anticorps Act5c, anticorps Actin, anticorps Actin/BAP47, anticorps Actin5C, anticorps BAP47, anticorps Bap47, anticorps CG4027, anticorps Dmel\\CG4027, anticorps M32055, anticorps T11, anticorps act, anticorps act 5C, anticorps act5C, anticorps actin, anticorps actin5C, anticorps anon-EST:fe2D2, anticorps beta-actin/Bap47, anticorps cyt5C, anticorps l(1)G0009, anticorps l(1)G0010, anticorps l(1)G0025, anticorps l(1)G0079, anticorps l(1)G0117, anticorps l(1)G0177, anticorps l(1)G0245, anticorps l(1)G0330, anticorps l(1)G0420, anticorps l(1)G0486, anticorps Bact, anticorps ACT-5, anticorps ACTB, anticorps B-ACTZF, anticorps actba, anticorps bact, anticorps bactin1, anticorps bactzf, anticorps wu:fd18f05, anticorps A26C1B, anticorps POTE2alpha, anticorps POTEACTIN, anticorps Beta-actin, anticorps actin beta, anticorps actin, beta, anticorps cytoplasmic actin OlCA1, anticorps beta-actin, anticorps Actin 5C, anticorps actin gamma 1, anticorps actin, beta 1, anticorps actin, beta L homeolog, anticorps beta actin, anticorps POTE ankyrin domain family member F, anticorps actin, beta like 2, anticorps actin, cytoplasmic 1, anticorps ACTB, anticorps Actb, anticorps actb, anticorps LOC100174883, anticorps LOC100304816, anticorps Act5C, anticorps ACTG1, anticorps actb1, anticorps actb.L, anticorps LOC100136352, anticorps POTEF, anticorps ACTBL2, anticorps LOC109073280

Sujet

This protein is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Alias Symbols: BRWS1, PS1TP5BP1
Protein Interaction Partner: FUS, DSTN, CAP2, CFL2, CFL1, ACTG1, ACTB, PA2G4, EHHADH, WDYHV1, HDAC6, ISG15, TP53, CEP250, TUBGCP2, TUBGCP3, VCP, UBC, TUBG1, SUMO2, LGALS3BP, AMOT, NEDD1, HAUS1, IVNS1ABP, STAU1, MDM2, CORO7, LGR4, ASB12, ASB9, ZBTB1, ERG, RPA3, RPA2, RPA1, CDKN2A, PAR
Protein Size: 375

Poids moléculaire

42 kDa

ID gène

60

NCBI Accession

NM_001101, NP_001092

UniProt

P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction