SF3A1 anticorps (N-Term)

N° du produit ABIN2778958

Détail du produit anti-SF3A1 anticorps

Antigène: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Cheval, Lapin, Cobaye, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SF3A1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Séquence: QQTTQQQLPQ KVQAQVIQET IVPKEPPPEF EFIADPPSIS AFDLDVVKLT

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Attributs du produit: This is a rabbit polyclonal antibody against SF3A1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human SF3A1

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 793 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-SF3A1 (ABIN2778958)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." dans: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Détails sur SF3A1

Antigène: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Autre désignation: SF3A1 (SF3A1 Produits)

Synonymes: anticorps Dmel\\CG16941, anticorps SF3a1, anticorps SF3a120, anticorps DDBDRAFT_0168938, anticorps DDBDRAFT_0233180, anticorps DDB_0168938, anticorps DDB_0233180, anticorps 1200014H24Rik, anticorps 5930416L09Rik, anticorps AI159724, anticorps si:dz150f13.2, anticorps wu:fc38e01, anticorps wu:fc50a11, anticorps wu:fj37c05, anticorps zgc:65786, anticorps PRP21, anticorps PRPF21, anticorps SAP114, anticorps SF3A120, anticorps CG16941 gene product from transcript CG16941-RA, anticorps SWAP/Surp domain-containing protein, anticorps splicing factor 3a, subunit 1, anticorps splicing factor 3a subunit 1, anticorps CG16941, anticorps sf3a1, anticorps spl1, anticorps Sf3a1, anticorps SF3A1

Sujet: SF3A1 is the subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family, named for the SURP motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs.This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family, named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.
Alias Symbols: PRP21, PRPF21, SAP114, SF3A120
Protein Interaction Partner: HDAC1, FUS, HUWE1, UBC, RPA3, RPA2, RPA1, WWOX, ZBTB1, SUZ12, EED, RNF2, rev, NR3C1, APBB1, HNRNPR, GAS7, CCDC6, PPP4R2, BRCC3, SMEK1, PRPF40A, GAPVD1, WBP4, TCERG1, HDAC11, HDAC6, SF3A3, DDX42, RBM10, SF3A2, SF1, WHSC1, IL7R, CSNK2A1, WDR77, RBFOX2, SMUR
Protein Size: 793

Poids moléculaire: 89 kDa

ID gène: 10291

NCBI Accession: NM_005877, NP_005868

UniProt: Q15459

Pathways: Ribonucleoprotein Complex Subunit Organization