NONO anticorps (N-Term)

N° du produit ABIN2779027

Détail du produit anti-NONO anticorps

Antigène: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Chien, Cheval, Boeuf (Vache), Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NONO est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Séquence: KQNHTPRKHH QHHHQQQHHQ QQQQQPPPPP IPANGQQASS QNEGLTIDLK

Homologie: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Attributs du produit: This is a rabbit polyclonal antibody against NONO. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human NONO

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 471 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-NONO (ABIN2779027)

Bubenik, Ladd, Gerber, Budiman, Driscoll: "Known turnover and translation regulatory RNA-binding proteins interact with the 3' UTR of SECIS-binding protein 2." dans: RNA biology, Vol. 6, Issue 1, pp. 73-83, (2009) (PubMed).

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." dans: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Détails sur NONO

Antigène: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Autre désignation: NONO (NONO Produits)

Synonymes: anticorps NMT55, anticorps NRB54, anticorps P54, anticorps P54NRB, anticorps nmt55, anticorps nrb54, anticorps p54nrb, anticorps xp54nrb, anticorps AA407051, anticorps AV149256, anticorps nonA, anticorps non-POU domain containing octamer binding, anticorps non-POU domain containing, octamer-binding, anticorps non-POU domain containing, octamer binding L homeolog, anticorps non-POU-domain-containing, octamer binding protein, anticorps NONO, anticorps Nono, anticorps nono.L

Sujet: NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
Alias Symbols: P54, NMT55, NRB54, P54NRB
Protein Interaction Partner: SFPQ, PTBP1, PRKAA2, PIN1, NONO, FUS, DDX6, C11orf68, PSPC1, HUWE1, LMO4, UBC, SUMO2, STAU1, MDM2, RPA3, RPA2, RPA1, ASB18, WWOX, ASB3, ERG, EED, rev, WBP4, TCERG1, APBB1, PRPF40A, LMNA, CD81, PAN2, CTNNB1, ORC5, MYC, ITGA4, IL7R, FN1, EWSR1, YLPM1, RNF43
Protein Size: 471

Poids moléculaire: 52 kDa

ID gène: 4841

NCBI Accession: NM_007363, NP_031389

UniProt: Q15233