PDHA1 anticorps (C-Term)

N° du produit ABIN2783236

Cet anticorps Lapin Polyclonal détecte spécifiquement PDHA1 dans WB. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Boeuf (Vache), Porc, Chien, Poisson zèbre (Danio rerio), Cobaye, Lapin et Cheval.

Aperçu rapide pour PDHA1 anticorps (C-Term) (ABIN2783236)

Antigène: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Chien, Poisson zèbre (Danio rerio), Cobaye, Lapin, Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDHA1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PDHA1 anticorps

Épitope: C-Term

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 82%

Attributs du produit: This is a rabbit polyclonal antibody against PDHA1. It was validated on Western Blot using a cell lysate as a positive control.

Immunogène: The immunogen is a synthetic peptide directed towards the C terminal region of human PDHA1

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 390 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur PDHA1

Antigène: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Autre désignation: PDHA1

Sujet: The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT, MIM 608770) (E2, EC 2.3.1.12), and dihydrolipoyl dehydrogenase (DLD, MIM 238331) (E3, EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: PDHA, PDHCE1A, PHE1A
Protein Interaction Partner: HUWE1, SUMO2, UBC, GINS4, DCPS, NCDN, VCL, PPP1R2, MSN, ASL, ADRB2, ITGA4, HNRNPDL, PDHX, SDHA, PDHB, OGDH, GTF2I, DLAT, CUL3, SIRT7, ATG101, WDR20, BABAM1, USP19, PDP1, EIF6, PDK4, PDK3, PDK2, PDK1, PDHA1, DGKE,
Protein Size: 390

Poids moléculaire: 40 kDa

ID gène: 5160

NCBI Accession: NM_000284, NP_000275

UniProt: P08559

Pathways: L'effet Warburg