OGT anticorps (N-Term)

N° du produit ABIN2783662

Cité dans 1 publication. Cet anticorps anti-OGT Polyclonal Lapin (ABIN2783662) détecte spécifiquement OGT dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache), Cheval, Lapin, Cobaye et Poisson zèbre (Danio rerio).

Aperçu rapide pour OGT anticorps (N-Term) (ABIN2783662)

Antigène: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Cheval, Lapin, Cobaye, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp OGT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-OGT anticorps

Épitope: N-Term

Séquence: ASSVGNVADS TEPTKRMLSF QGLAELAHRE YQAGDFEAAE RHCMQLWRQE

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Attributs du produit: This is a rabbit polyclonal antibody against OGT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human OGT

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 1046 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-OGT (ABIN2783662)

Taylor, Parker, Hazel, Soesanto, Fuller, Yazzie, McClain: "Glucose deprivation stimulates O-GlcNAc modification of proteins through up-regulation of O-linked N-acetylglucosaminyltransferase." dans: The Journal of biological chemistry, Vol. 283, Issue 10, pp. 6050-7, (2008) (PubMed).

Détails sur OGT

Antigène: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Autre désignation: OGT

Sujet: OGT catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: FLJ23071, HRNT1, MGC22921, O-GLCNAC
Protein Interaction Partner: FBXW11, HUWE1, UBC, PSG1, PHC3, NUP62CL, TET2, SUMO1, NEDD8, WWOX, BAP1, P3H1, RPRD1B, PPME1, LAP3, DBNL, HSPBP1, GPN1, WDR4, ACTR2, TOM1L1, ZPR1, SURF2, LPP, AGFG1, ALAD, C14orf142, UBL4A, FOXO4, HCFC1, SMURF1, Hoxa1, Nfatc1, Nup62, RELA, PPP1CB, PPP1CA,
Protein Size: 1046

Poids moléculaire: 117 kDa

ID gène: 8473

NCBI Accession: NM_181672, NP_858058

UniProt: O15294

Pathways: Regulation of Carbohydrate Metabolic Process