CCDC42 anticorps (Middle Region)
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- Antigène Voir toutes CCDC42 Anticorps
- CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))
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Épitope
- Middle Region
- Reactivité
- Humain, Souris, Rat, Cobaye, Cheval, Lapin, Boeuf (Vache), Chien
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp CCDC42 est non-conjugé
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Application
- Western Blotting (WB)
- Séquence
- ENSEFEEIHE VIARYKTLVS MHHDLMQSAQ EGQEKIEHAK ARLARYMEEK
- Homologie
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 93%
- Attributs du produit
- This is a rabbit polyclonal antibody against Ccdc42. It was validated on Western Blot.
- Purification
- Affinity Purified
- Immunogène
- The immunogen is a synthetic peptide directed towards the middle region of Rat Ccdc42
- Top Product
- Discover our top product CCDC42 Anticorps primaire
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- Indications d'application
- Optimal working dilutions should be determined experimentally by the investigator.
- Commentaires
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Antigen size: 316 AA
- Restrictions
- For Research Use only
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- by
- Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University of Göttingen
- No.
- #103398
- Date
- 12.10.2018
- Antigène
- CCDC42
- Numéro du lot
- QC28887-41199
- Application validée
- Immunocytochemistry
- Contrôle positif
- NIH/3T3 cells transfected with Cddc42-mCherry expression vector
- Contrôle négative
- Non-transfected NIH/3T3 cells
- Conclusion
Passed. ABIN2785068 specifically detects Ccdc42-Cherry when exogenously expressed. Background staining is low.
- Anticorps primaire
- ABIN2785068
- Anticorps secondaire
- goat anti-rabbit 488 antibody (MoBiotec, MFP-A1008, lot 2806F058)
- Full Protocol
- Grow NIH/3T3 cells (ATCC, CRL-1658) in DMEM+GlutaMAX (Gibco, 10567-014, lot 1922818) supplemented with fetal bovine serum (Gibco, 10500-064, lot 08FO477K) and Pen/Strep (Gibco, 15140-122, lot 1924797) at 37°C and 5% CO2 to 70% confluency.
- Transfect cells with Ccdc42 tagged mCherry (Ccdc42-Cherry) expression vector using EndofectinMax (GeneCopoeia) following the manufacturer´s instructions.
- Grow cells for 24h.
- Fix cells in methanol for 10min at -20°C followed by incubation in 0.3% Triton X-100 for 10min at 4°C.
- Block cells in PBS containing 1% bovine serum albumin and 0.5% Tween-20 (PBT) for 1h at RT.
- Incubate cells with primary rabbit anti-Ccdc42 antibody (antibodies-online, ABIN2785068, QC28887-41199) diluted 1:100 in PBS for 1h at 37°C.
- Wash cells 3x 5min with 1x PBS.
- Incubate cells with secondary goat anti-rabbit 488 antibody (MoBiotec, MFP-A1008, lot 2806F058) diluted 1:300 for 1h at 37°C in PBS.
- DNA was counterstained with DAPI (4’, 6-Diamidino-2-phenylindole; Sigma, D-9542).
- Image acquisition on Zeiss LSM 510 confocal microscope and processing using Adobe Photoshop 5.0.
- Notes
Validation #103398 (Immunocytochemistry)Validation ImagesProtocole -
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -20 °C
- Stockage commentaire
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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-
Essential Role of CFAP53 in Sperm Flagellum Biogenesis." dans: Frontiers in cell and developmental biology, Vol. 9, pp. 676910, (2021) (PubMed).
: "CCDC42 Localizes to Manchette, HTCA and Tail and Interacts With ODF1 and ODF2 in the Formation of the Male Germ Cell Cytoskeleton." dans: Frontiers in cell and developmental biology, Vol. 7, pp. 151 (PubMed).
: "
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Essential Role of CFAP53 in Sperm Flagellum Biogenesis." dans: Frontiers in cell and developmental biology, Vol. 9, pp. 676910, (2021) (PubMed).
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- Antigène
- CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))
- Autre désignation
- Ccdc42 (CCDC42 Produits)
- Synonymes
- anticorps RGD1565925, anticorps CCDC42A, anticorps A530001H01Rik, anticorps coiled-coil domain containing 42, anticorps Ccdc42, anticorps CCDC42
- Sujet
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The function of this protein remains unknown.
Alias Symbols: RGD1565925
Protein Size: 316 - Poids moléculaire
- 34 kDa
- ID gène
- 303234
- NCBI Accession
- NM_001107009, NP_001100479
- UniProt
- D3ZAZ8
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