CCDC42 anticorps (Middle Region)

N° du produit ABIN2785068

Détail du produit anti-CCDC42 anticorps

Antigène: CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))

Épitope: Middle Region

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CCDC42 est non-conjugé

Application: Western Blotting (WB)

Séquence: ENSEFEEIHE VIARYKTLVS MHHDLMQSAQ EGQEKIEHAK ARLARYMEEK

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 93%

Attributs du produit: This is a rabbit polyclonal antibody against Ccdc42. It was validated on Western Blot.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of Rat Ccdc42

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 316 AA

Restrictions: For Research Use only

Independent Validation (ICC)

Validation #103398 (Immunocytochemistry)

by: Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University of Göttingen

No.: #103398

Date: 12.10.2018

Antigène: CCDC42

Numéro du lot: QC28887-41199

Application validée: Immunocytochemistry

Contrôle positif: NIH/3T3 cells transfected with Cddc42-mCherry expression vector

Contrôle négative: Non-transfected NIH/3T3 cells

Conclusion:

Passed. ABIN2785068 specifically detects Ccdc42-Cherry when exogenously expressed. Background staining is low.

Validation Images

Cells were transfected with Ccdc42 tagged with mCherry (red) and incubated with ABIN2785068 (green). The pictures on the right show the red and green channels merged with DAPI counterstain (blue).

Protocole

Anticorps primaire: ABIN2785068

Anticorps secondaire: goat anti-rabbit 488 antibody (MoBiotec, MFP-A1008, lot 2806F058)

Full Protocol:

• Grow NIH/3T3 cells (ATCC, CRL-1658) in DMEM+GlutaMAX (Gibco, 10567-014, lot 1922818) supplemented with fetal bovine serum (Gibco, 10500-064, lot 08FO477K) and Pen/Strep (Gibco, 15140-122, lot 1924797) at 37°C and 5% CO₂ to 70% confluency.
• Transfect cells with Ccdc42 tagged mCherry (Ccdc42-Cherry) expression vector using EndofectinMax (GeneCopoeia) following the manufacturer´s instructions.
• Grow cells for 24h.
• Fix cells in methanol for 10min at -20°C followed by incubation in 0.3% Triton X-100 for 10min at 4°C.
• Block cells in PBS containing 1% bovine serum albumin and 0.5% Tween-20 (PBT) for 1h at RT.
• Incubate cells with primary rabbit anti-Ccdc42 antibody (antibodies-online, ABIN2785068, QC28887-41199) diluted 1:100 in PBS for 1h at 37°C.
• Wash cells 3x 5min with 1x PBS.
• Incubate cells with secondary goat anti-rabbit 488 antibody (MoBiotec, MFP-A1008, lot 2806F058) diluted 1:300 for 1h at 37°C in PBS.
• DNA was counterstained with DAPI (4’, 6-Diamidino-2-phenylindole; Sigma, D-9542).
• Image acquisition on Zeiss LSM 510 confocal microscope and processing using Adobe Photoshop 5.0.

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-CCDC42 (ABIN2785068)

Wu, Yu, Liu, Wang, Huang, Lu, Chen, Li, Liu: "Essential Role of CFAP53 in Sperm Flagellum Biogenesis." dans: Frontiers in cell and developmental biology, Vol. 9, pp. 676910, (2021) (PubMed).

Tapia Contreras, Hoyer-Fender: "CCDC42 Localizes to Manchette, HTCA and Tail and Interacts With ODF1 and ODF2 in the Formation of the Male Germ Cell Cytoskeleton." dans: Frontiers in cell and developmental biology, Vol. 7, pp. 151 (PubMed).

Détails sur CCDC42

Antigène: CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))

Autre désignation: Ccdc42 (CCDC42 Produits)

Synonymes: anticorps RGD1565925, anticorps CCDC42A, anticorps A530001H01Rik, anticorps coiled-coil domain containing 42, anticorps Ccdc42, anticorps CCDC42

Sujet: The function of this protein remains unknown.
Alias Symbols: RGD1565925
Protein Size: 316

Poids moléculaire: 34 kDa

ID gène: 303234

NCBI Accession: NM_001107009, NP_001100479

UniProt: D3ZAZ8