PRAME anticorps (N-Term)

N° du produit ABIN2786465

Détail du produit anti-PRAME anticorps

Antigène: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cheval, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRAME est non-conjugé

Application: Western Blotting (WB)

Séquence: MERRRLWGSI QSRYISMSVW TSPRRLVELA GQSLLKDEAL AIAALELLPR

Homologie: Cow: 86%, Horse: 93%, Human: 100%, Mouse: 79%, Pig: 86%, Rat: 85%

Attributs du produit: This is a rabbit polyclonal antibody against PRAME. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human PRAME

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 509 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-PRAME (ABIN2786465)

Liu, Zhao, Lu, Ning, Ma, Diaz, OConnor: "A novel testis-specific protein, PRAMEY, is involved in spermatogenesis in cattle." dans: Reproduction (Cambridge, England), Vol. 153, Issue 6, pp. 847-863, (2017) (PubMed).

Détails sur PRAME

Antigène: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Autre désignation: PRAME (PRAME Produits)

Sujet: PRAME functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. PRAME prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene.
Alias Symbols: MAPE, OIP4, CT130
Protein Interaction Partner: PRAME, RBX1, CUL2, TCEB2, TCEB1, ZNF408, FBXL12, XRN2, HIST4H4, HIST2H2BE, HIST2H2AC, HIST3H3, UBC, RARA, TP53RK, OSGEP, TPRKB, KDM1A, BAG2, LAGE3, UBL4A, SNRPB, RPS13, RPL22, RPL10, RFC4, PSMC4, HNRNPM, HNRNPH1, HNRNPF, HNRNPC, NEDD8, STK19,
Protein Size: 509

Poids moléculaire: 56 kDa

ID gène: 23532

NCBI Accession: NM_006115, NP_006106

UniProt: P78395

Pathways: Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding