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ARHGEF4 anticorps (N-Term)

ARHGEF4 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin WB Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN2787993
  • Antigène Voir toutes ARHGEF4 Anticorps
    ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))
    Épitope
    • 8
    • 7
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin
    Hôte
    • 25
    • 5
    • 1
    Lapin
    Clonalité
    • 27
    • 4
    Polyclonal
    Conjugué
    • 11
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp ARHGEF4 est non-conjugé
    Application
    • 23
    • 17
    • 4
    • 1
    Western Blotting (WB)
    Séquence
    ADDYEAPRAG AREADDSGPE AQSCKDQMRT NVINEILSTE RDYIKHLRDI
    Homologie
    Cow: 79%, Dog: 93%, Guinea Pig: 92%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%
    Attributs du produit
    This is a rabbit polyclonal antibody against Arhgef4. It was validated on Western Blot.
    Purification
    Affinity Purified
    Immunogène
    The immunogen is a synthetic peptide directed towards the N-terminal region of Mouse Arhgef4
  • Indications d'application
    Optimal working dilutions should be determined experimentally by the investigator.
    Commentaires

    Antigen size: 484 AA

    Restrictions
    For Research Use only
  • Validation #100106 (Western Blotting)
    'Independent Validation' signe
    by
    Instituto de Parasitología y Biomedicina López-Neyra
    No.
    #100106
    Date
    23.12.2016
    Antigène
    ARHGEF4
    Numéro du lot
    QC30159-41213
    Application validée
    Western Blotting
    Contrôle positif
    primary mouse microglia
    Contrôle négative
    Conclusion
    Passed. ABIN2787993 specifically recognizes Arhgef4 in primary mouse microglia lysates.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN2787993
    Anticorps secondaire
    anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)
    Full Protocol
    • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
    • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, protease inhibitors (Sigma)).
    • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
    • Denature 5µg, 10µg and 30µg total protein for 5min at 95°C in 5µl, 10µl and 20µl Laemmli SDS sample buffer and subsequently separate them on a denaturing self-made 10% SDS-PAGE for 1h at 140V.
    • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
    • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk for 1h at RT.
    • Wash membrane 3x 5min with TBST.
    • Incubation with primary rabbit anti-ARHGEF4 antibody (antibodies-online, ABIN2787993, lot QC30159-41213) diluted 1:500 in TBST containing 2% BSA ON at 4°C.
    • Wash membrane 5x 5min with TBST.
    • Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
    • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
    • Reveal protein bands using ECL Plus Western Blotting Detection Reagent (GE Healthcare, RPN2132, lot 4645641) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
    Notes
    The ARHGEF4 antibody ABIN2787993 reveals multiple bands as is expected for murine Arhgef4.
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Antigène
    ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))
    Autre désignation
    Arhgef4 (ARHGEF4 Produits)
    Synonymes
    anticorps ARHGEF4, anticorps ASEF, anticorps ASEF1, anticorps GEF4, anticorps STM6, anticorps 9330140K16Rik, anticorps Asef, anticorps ENSMUSG00000070955, anticorps Rho guanine nucleotide exchange factor 4, anticorps uncharacterized LOC100470646, anticorps rho guanine nucleotide exchange factor 4, anticorps Rho guanine nucleotide exchange factor (GEF) 4, anticorps ARHGEF4, anticorps arhg4, anticorps LOC100470646, anticorps LOC100544917, anticorps Arhgef4
    Sujet
    Arhgef4 acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion By similarity. It is required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Arhgef4 is involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
    Alias Symbols: 9330140K16Rik, Asef, ENSMUSG00000070955
    Protein Size: 484
    Poids moléculaire
    57 kDa
    ID gène
    226970
    NCBI Accession
    NM_183019, NP_898840
    UniProt
    Q7TNR9
    Pathways
    Neurotrophin Signaling Pathway
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