GCM1 anticorps (N-Term)

N° du produit ABIN2792608

Détail du produit anti-GCM1 anticorps

Antigène: GCM1 (Glial Cells Missing Homolog 1 (GCM1))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GCM1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Séquence: MEPDDFDSED KEILSWDIND VKLPQNVKKT DWFQEWPDSY AKHIYSSEDK

Réactivité croisée: Chien, Humain, Souris, Rat (Rattus)

Homologie: Dog: 78%, Human: 92%, Mouse: 78%, Rat: 78%

Attributs du produit: This is a rabbit polyclonal antibody against GCM1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human GCM1

Information d'application

Indications d'application: WB Suggested Anti-GCM1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:7812500
Positive Control: Human Placenta.
Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 436 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-GCM1 (ABIN2792608)

Bailey, Alahari, Tagliaferro, Post, Caniggia: "Augmented trophoblast cell death in preeclampsia can proceed via ceramide-mediated necroptosis." dans: Cell death & disease, Vol. 8, Issue 2, pp. e2590, (2017) (PubMed).

Moretto Zita, Soncin, Natale, Pizzo, Parast: "Gene Expression Profiling Reveals a Novel Regulatory Role for Sox21 Protein in Mouse Trophoblast Stem Cell Differentiation." dans: The Journal of biological chemistry, Vol. 290, Issue 50, pp. 30152-62, (2015) (PubMed).

Sivasubramaniyam, Garcia, Tagliaferro, Melland-Smith, Chauvin, Post, Todros, Caniggia: "Where polarity meets fusion: role of Par6 in trophoblast differentiation during placental development and preeclampsia." dans: Endocrinology, Vol. 154, Issue 3, pp. 1296-309, (2013) (PubMed).

Kumar, Luo, Tudela, Alexander, Mendelson: "The c-Myc-regulated microRNA-17~92 (miR-17~92) and miR-106a~363 clusters target hCYP19A1 and hGCM1 to inhibit human trophoblast differentiation." dans: Molecular and cellular biology, Vol. 33, Issue 9, pp. 1782-96, (2013) (PubMed).

Drewlo, Czikk, Baczyk, Lye, Kingdom: "Glial cell missing-1 mediates over-expression of tissue inhibitor of metalloproteinase-4 in severe pre-eclamptic placental villi." dans: Human reproduction (Oxford, England), Vol. 26, Issue 5, pp. 1025-34, (2011) (PubMed).

Baczyk, Drewlo, Proctor, Dunk, Lye, Kingdom: "Glial cell missing-1 transcription factor is required for the differentiation of the human trophoblast." dans: Cell death and differentiation, Vol. 16, Issue 5, pp. 719-27, (2009) (PubMed).

Détails sur GCM1

Antigène: GCM1 (Glial Cells Missing Homolog 1 (GCM1))

Autre désignation: GCM1 / GCMA (GCM1 Produits)

Synonymes: anticorps GCM1, anticorps GCM, anticorps GCM-1, anticorps GCMA, anticorps hGCMa, anticorps GCMa, anticorps Gcm-rs2, anticorps Gcm1-rs1, anticorps glide, anticorps Gcma, anticorps glial cells missing homolog 1, anticorps glial cells missing homolog 1 (Drosophila), anticorps GCM1, anticorps Gcm1

Sujet: GCM1 is a DNA-binding protein with a gcm-motif (glial cell missing motif). The encoded protein is a homolog of the Drosophila glial cells missing gene (gcm). This protein binds to the GCM-motif (A/G)CCCGCAT, a novel sequence among known targets of DNA-binding proteins. The N-terminal DNA-binding domain confers the unique DNA-binding activity of this protein.This gene encodes a DNA-binding protein with a gcm-motif (glial cell missing motif). The encoded protein is a homolog of the Drosophila glial cells missing gene (gcm). This protein binds to the GCM-motif (A/G)CCCGCAT, a novel sequence among known targets of DNA-binding proteins. The N-terminal DNA-binding domain confers the unique DNA-binding activity of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-132 D88613.1 1-132 133-2763 AB026493.1 1-2631
Alias Symbols: GCMA, hGCMa
Protein Interaction Partner: FBXW2, UBC, SENP1, CAMK1, SUMO1, DUSP23, CREBBP, HDAC5, HDAC4, HDAC3, HDAC1, CUL1, SKP1, UBE2I,
Protein Size: 436

Poids moléculaire: 49 kDa

ID gène: 8521

NCBI Accession: NM_003643, NP_003634

UniProt: Q9NP62