PCNA anticorps

N° du produit ABIN2854788

Détail du produit anti-PCNA anticorps

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Fluorescence in situ hybridization (FISH)

Specificité: IP/MS validation was supported by references (PMID:30377373)

Réactivité croisée: Hamsters dorés syriens, Humain, Souris, Rat

Attributs du produit: Rabbit Polyclonal antibody to PCNA (proliferating cell nuclear antigen)
PCNA antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: NIH-3T3 , PC-12

Validation: IP/MS, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.11 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-PCNA (ABIN2854788)

Mousa, Alhumaydhi, Abdellatif, Abdulmonem, AlKhowailed, Alsagaby, Al Rugaie, Alnuqaydan, Aljohani, Aljasir, Alwashmi, Soliman, Yosof, Elsheikh, Babiker, Alsuhaibani, Hegazy, Seleem: "Curcumin and ustekinumab cotherapy alleviates induced psoriasis in rats through their antioxidant, anti-inflammatory, and antiproliferative effects." dans: Cutaneous and ocular toxicology, pp. 1-10, (2021) (PubMed).

Mousa, Soliman, Alhumaydhi, Almatroudi, Al Rugaie, Allemailem, Alrumaihi, Khan, Rezk, Aljasir, Alwashmi, Aba Alkhayl, Albutti, Seleem: "Garlic Extract Alleviates Trastuzumab-Induced Hepatotoxicity in Rats Through Its Antioxidant, Anti-Inflammatory, and Antihyperlipidemic Effects." dans: Journal of inflammation research, Vol. 14, pp. 6305-6316, (2021) (PubMed).

Chen, Chen, Lin, Hsieh, Hsu, Hsieh: "Synergistic Anticancer Effects of Gemcitabine with Pitavastatin on Pancreatic Cancer Cell Line MIA PaCa-2 in vitro and in vivo." dans: Cancer management and research, Vol. 12, pp. 4645-4665, (2020) (PubMed).

Hsieh, Wang, Changou, Chin, Yang, Lai, Lee, Yang, Whang-Peng, Liu, Lin, Mousa, Davis: "Crosstalk between integrin αvβ3 and ERα contributes to thyroid hormone-induced proliferation of ovarian cancer cells." dans: Oncotarget, Vol. 8, Issue 15, pp. 24237-24249, (2018) (PubMed).

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: proliferating cell nuclear antigen (PCNA Produits)

Synonymes: anticorps PCNAR, anticorps Pcna/cyclin, anticorps pcna, anticorps AO090003000846, anticorps 53/13, anticorps CG9193, anticorps DmPCNA, anticorps Dmel\\CG9193, anticorps MUS209, anticorps Mus209, anticorps PCNA, anticorps PCNA/mus209, anticorps Pcna, anticorps dPCNA, anticorps l(2)02448, anticorps l(2)56F, anticorps l(2)56Fa, anticorps l(2)s1534, anticorps cb16, anticorps fb36g03, anticorps wu:fa28e03, anticorps wu:fb36g03, anticorps pcna-A, anticorps proliferating cell nuclear antigen, anticorps DNA polymerase III sliding clamp, anticorps Proliferating cell nuclear antigen, anticorps Proliferating cell nuclear antigen-like, anticorps proliferating cell nuclear antigen L homeolog, anticorps proliferating cell nuclear antigen 1, anticorps PCNA, anticorps Pcna, anticorps pcna, anticorps MMP_RS08810, anticorps ANI_1_512014, anticorps AOR_1_1500154, anticorps MGC53867, anticorps pcna.L, anticorps pcna1, anticorps LOC106420145, anticorps pcn1

Sujet: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Cellular Localization: Nucleus

Poids moléculaire: 29 kDa

ID gène: 5111

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy