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Vimentin anticorps

Name: Vimentin anticorps
Brand: antibodies-online
SKU: ABIN2854814
Price: 630.63 EUR
Availability: InStock

KO Validated VIM Reactivité: Humain WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Hôte: Lapin Polyclonal unconjugated

WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody , diluted at 1:10000. (Vimentin anticorps)

WB Image Vimentin antibody detects Vimentin protein by western blot analysis. (Vimentin anticorps)

IHC-P Image Vimentin antibody detects Vimentin protein at cytoplasm in rat testis by immunohistochemical analysis. (Vimentin anticorps)

IHC

IP Image Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5 μg of Vimentin antibody, Western blot analysis was performed using Vimentin antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (Vimentin anticorps)

IHC-P Image Vimentin antibody detects Vimentin protein at cytosol on mouse thymus gland by immunohistochemical analysis. (Vimentin anticorps)

IHC

IHC-Fr Image Immunofluorescence photomicrographs of frozen sections of mouse brain. (Vimentin anticorps)

IHC

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Vimentin, antibody (Green) at 1:500 dilution. (Vimentin anticorps)

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Vimentin, antibody at 1:500 dilution. (Vimentin anticorps)

IHC

Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells. (Vimentin anticorps)

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin. (Vimentin anticorps)

Fusion progeny exhibit epithelial-mesenchymal transition(A) Representative phase-contrast microscopic images of fusion progeny and parental cells. (Vimentin anticorps)

FOXF1 mediates reprogramming effects on fusion progeny(A) FOXF1 mRNA levels were determined by real-time PCR in fusion progeny and parent cell lines. (Vimentin anticorps)

Expression pattern of Notch ligands and receptors in first trimester decidual tissue. (Vimentin anticorps)

IF (cc)

MEF2B-V5 expression alters the abundance of protein from target genes and increases HEK293A cell migration. (Vimentin anticorps)

MEF2B mutations alter the abundance of protein from MEF2B target genes and decrease cell migration. (Vimentin anticorps)

Serum-free suspension culture induces the enrichment of Glioma stem-like cells (GSCs). (Vimentin anticorps)

Aryl hydrocarbon receptor (AhR) expression in different cells was correlated with E-cadherin and vimentin expression and cell motility. (Vimentin anticorps)

Level of autophagy-related protein expression in different cells was correlated with their cell motility. (Vimentin anticorps)

Expression of epithelial-mesenchymal transition (EMT) markers in MCF-7 o/e Snail cells. (Vimentin anticorps)

Differential expression of epithelial-mesenchymal transition (EMT) markers in MCF-7 o/e Snail cells and MCF-F o/e Snail tissues. (Vimentin anticorps)

ZEB2-AS1 knockdown inhibits migration, invasion and EMT in HNSCC cells. (Vimentin anticorps)

Reintroduction of exogenous ZEB2 largely attenuated the phenotypical changes induced by ZEB2-AS1 inhibition. (Vimentin anticorps)

ZEB2-AS1 is involved for TGF-β1-induced EMT in HNSCC cells. (Vimentin anticorps)

Lipid loading augments hypoxia mediated secretion of pro-tumorigenic factors. (Vimentin anticorps)

ISX expression induces TWIST1 and Snail1 expression, and promotes EMTAThe expression levels of ISX, TWIST1, Snail1, fibronectin (FN1), VEGF, and E-cadherin (CDH1) mRNA were examined in a ISX-GFP inducible Tet-ON transformants at 8 h after addition of DOX. (Vimentin anticorps)

ISX interacts with both BRD4 and PCAF in vitro and in vivoABRD4 peptide (291-314 a. (Vimentin anticorps)

The expression of ISX, BRD4, and PCAF is correlated with lung cancer metastasisAIHC staining with ISX (brown) or PCAF (brown) antibody in lung tumors from patients with lung cancer. (Vimentin anticorps)

Sequencing analysis of acetylated recombinant and trypsin-digested wild-type ISX using liquid chromatography-mass spectrometryA, BPCAF acetylation of ISX was detected at lysine residue 69 by liquid chromatography-tandem mass spectrometry (LC-tandem MS-MS). (Vimentin anticorps)

Cataracts induced epithelial-mesenchymal transition (EMT) progression with significantly elevated N-cadherin and MMP9 expression in DM (+) patients. (Vimentin anticorps)

IF (cc)

35 images

(17 références)

N° du produit ABIN2854814

Fiche de données

Antigène Voir toutes Vimentin (VIM) Anticorps

Vimentin (VIM)
Reactivité
342

135

132

61

60

50

43

38

30

22

20

14

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Humain
Hôte
194

131

11

6

6

5

2
Lapin
Anticorps secondaires appropriés
Clonalité
210

144
Polyclonal
Conjugué
204

19

14

14

10

7

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
Cet anticorp Vimentin est non-conjugé
Application
286

130

106

99

96

63

45

40

39

32

23

18

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée

Cat, Chien, Humain, Souris, Rat

Attributs du produit

Rabbit Polyclonal antibody to Vimentin (vimentin)
Vimentin antibody

Purification

Purified by antigen-affinity chromatography.

Classe de qualité

KO Validated

Immunogène

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Isotype

IgG

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anti-Vimentin (VIM) (AA 2-466) antibody
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anti-Vimentin (VIM) (AA 41-140) antibody
VIM Reactivité: Humain WB, ELISA, IF, IP Hôte: Souris Monoclonal 3E9 unconjugated

Indications d'application

WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires

Positive Control: 293T
Validation: Comparison, KO/KD, Orthogonal, Overexpression

Restrictions

For Research Use only
Format

Liquid

Concentration

0.11 mg/mL

Buffer

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Navarrete-Bernal, Cervantes-Badillo, Martínez-Herrera, Lara-Torres, Gerson-Cwilich, Zentella-Dehesa, Ibarra-Sánchez, Esparza-López, Montesinos, Cortés-Morales, Osorio-Pérez, Villegas-Osorno et al.: "Biological Landscape of Triple Negative Breast Cancers Expressing CTLA-4. ..." dans: Frontiers in oncology, Vol. 10, pp. 1206, (2020) (PubMed).

Klein, Lohr, Droste: "Age-Dependent Heterogeneity of Murine Olfactory Bulb Astrocytes." dans: Frontiers in aging neuroscience, Vol. 12, pp. 172, (2020) (PubMed).

Horn, Riskin, Hempel, Fousek, Lind, Hamilton, McCampbell, Maeda, Zebala, Su, Schlom, Palena: "Simultaneous inhibition of CXCR1/2, TGF-β, and PD-L1 remodels the tumor and its microenvironment to drive antitumor immunity." dans: Journal for immunotherapy of cancer, Vol. 8, Issue 1, (2020) (PubMed).

Wang, Liu, Jeng, Chiang, Chai, Chiou, Huang, Yokoyama, Wang, Huang, Hsu: "PCAF-mediated acetylation of ISX recruits BRD4 to promote epithelial-mesenchymal transition." dans: EMBO reports, Vol. 21, Issue 2, pp. e48795, (2020) (PubMed).

Diao, Ge, Song, Wu, Li, Li, Yang, Wang, Cheng: "Overexpression of ZEB2-AS1 promotes epithelial-to-mesenchymal transition and metastasis by stabilizing ZEB2 mRNA in head neck squamous cell carcinoma." dans: Journal of cellular and molecular medicine, Vol. 23, Issue 6, pp. 4269-4280, (2020) (PubMed).

Xie, Zhao, Guo, Liu, Fu, Fan, Lin, Chen, Wang, Fan, Wei: "Benzyl isothiocyanate suppresses development and metastasis of murine mammary carcinoma by regulating the Wnt/β‑catenin pathway." dans: Molecular medicine reports, Vol. 20, Issue 2, pp. 1808-1818, (2019) (PubMed).

Offer, Menard, Pérez, de Oliveira, Chandran, Johansson, Bång-Rudenstam, Siesjö, Ebbesson, Hedenfalk, Sundgren, Darabi, Belting: "Extracellular lipid loading augments hypoxic paracrine signaling and promotes glioma angiogenesis and macrophage infiltration." dans: Journal of experimental & clinical cancer research : CR, Vol. 38, Issue 1, pp. 241, (2019) (PubMed).

Hua, Liu, Hsu, Cheng, Yang, Chang, Lee, Hsiao, Juan, Chien, Yang: "3'UTR polymorphisms of carbonic anhydrase IX determine the miR-34a targeting efficiency and prognosis of hepatocellular carcinoma." dans: Scientific reports, Vol. 7, Issue 1, pp. 4466, (2019) (PubMed).

Chu, Lee, Lee, Wang, Huang, Lan, Lin, Lin, Tian, Liu, Chow: "MiR-338-5p promotes metastasis of colorectal cancer by inhibition of phosphatidylinositol 3-kinase, catalytic subunit type 3-mediated autophagy pathway." dans: EBioMedicine, Vol. 43, pp. 270-281, (2019) (PubMed).

Nakazawa, Taniyama, Sanada, Morishita, Nakamori, Morimoto, Yeung, Yang: "Periostin blockade overcomes chemoresistance via restricting the expansion of mesenchymal tumor subpopulations in breast cancer." dans: Scientific reports, Vol. 8, Issue 1, pp. 4013, (2019) (PubMed).

Chiu, Wen, Wang, Hsu, Tsai, Hung, Tseng, Shyr: "Role of estrogen receptors and Src signaling in mechanisms of bone metastasis by estrogen receptor positive breast cancers." dans: Journal of translational medicine, Vol. 15, Issue 1, pp. 97, (2018) (PubMed).

Wang, Wu, Yeh, Su, Hsia, Liang, Chen, Hsueh, Chen: "Melatonin suppresses hepatocellular carcinoma progression via lncRNA-CPS1-IT-mediated HIF-1α inactivation." dans: Oncotarget, Vol. 8, Issue 47, pp. 82280-82293, (2017) (PubMed).

Hsu, Chang, Hsu, Liu, Yeh, Wang, Liou, Ko, Chang, Chuang: "Suberoylanilide hydroxamic acid represses glioma stem-like cells." dans: Journal of biomedical science, Vol. 23, Issue 1, pp. 81, (2017) (PubMed).

Yin, Yin, Wang, Zhou, Liu, Yang, Wang, Lu: "MiR-206 suppresses epithelial mesenchymal transition by targeting TGF-β signaling in estrogen receptor positive breast cancer cells." dans: Oncotarget, Vol. 7, Issue 17, pp. 24537-48, (2017) (PubMed).

Marquez-Vilendrer, Rai, Gramling, Lu, Reisman: "Loss of the SWI/SNF ATPase subunits BRM and BRG1 drives lung cancer development." dans: Oncoscience, Vol. 3, Issue 11-12, pp. 322-336, (2016) (PubMed).

Hou, You, Yang, Pan, Chen, Lee, Lin, Liou, Liu, Chi, Ger, Tsai: "Aberrant DNA hypomethylation of miR-196b contributes to migration and invasion of oral cancer." dans: Oncology letters, Vol. 11, Issue 6, pp. 4013-4021, (2016) (PubMed).

Wei, Nickoloff, Chen, Liu, Lo, Chang, Yang, Wu, Williams, Gelovani, Deng: "FOXF1 mediates mesenchymal stem cell fusion-induced reprogramming of lung cancer cells." dans: Oncotarget, Vol. 5, Issue 19, pp. 9514-29, (2015) (PubMed).
Antigène

Vimentin (VIM)

Autre désignation

vimentin (VIM Produits)

Synonymes

anticorps CTRCT30, anticorps cb28, anticorps vime, anticorps vim, anticorps vim1, anticorps vim2, anticorps VIM, anticorps Vimentin, anticorps vim4, anticorps vimentin, anticorps vimentin L homeolog, anticorps vimentin S homeolog, anticorps VIM, anticorps Vim, anticorps vim, anticorps vim.L, anticorps vim.S

Sujet

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Poids moléculaire

54 kDa

ID gène

7431

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis