KAP1 anticorps

N° du produit ABIN2855331

Détail du produit anti-KAP1 anticorps

Antigène: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KAP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Réactivité croisée: Humain, Souris

Attributs du produit: Rabbit Polyclonal antibody to KAP1 (tripartite motif containing 28)
KAP1 antibody [N3C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: NIH-3T3

Validation: Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.48 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-KAP1 (ABIN2855331)

Liu, Li, Liu, Li, Wei, Liu, Lv: "Expression and Significance of TRIM 28 in Squamous Carcinoma of Esophagus." dans: Pathology oncology research : POR, Vol. 25, Issue 4, pp. 1645-1652, (2020) (PubMed).

Hung, Johnson, Chen, Byrum, Bredemeyer, Yewdell, Johnson, Lee, Deivasigamani, Hindi, Amatya, Gross, Paull, Pisapia, Chaudhuri, Petrini, Mosammaparast, Amarasinghe, Zha, Tyler, Sleckman: "MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining." dans: Molecular cell, Vol. 71, Issue 2, pp. 332-342.e8, (2019) (PubMed).

Wang, Wolgemuth: "BET Protein BRDT Complexes With HDAC1, PRMT5, and TRIM28 and Functions in Transcriptional Repression During Spermatogenesis." dans: Journal of cellular biochemistry, Vol. 117, Issue 6, pp. 1429-38, (2016) (PubMed).

Aslanian, Yates, Hunter: "Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells." dans: DNA repair, Vol. 15, pp. 29-38, (2014) (PubMed).

Détails sur KAP1

Antigène: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Autre désignation: tripartite motif containing 28 (TRIM28 Produits)

Synonymes: anticorps KAP1, anticorps RNF96, anticorps TF1B, anticorps TIF1B, anticorps TRIM28, anticorps kap1, anticorps rnf96, anticorps tf1b, anticorps tif1b, anticorps AA408787, anticorps KAP-1, anticorps KRIP-1, anticorps Tif1b, anticorps Tif1beta, anticorps Kap1, anticorps Krip1, anticorps TIF1BETA, anticorps tripartite motif containing 28, anticorps tripartite motif containing 28 L homeolog, anticorps tripartite motif-containing 28, anticorps TRIM28, anticorps trim28.L, anticorps Trim28

Sujet: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Cellular Localization: Nucleus (By similarity)

Poids moléculaire: 89 kDa

ID gène: 10155

UniProt: Q13263

Pathways: Signalisation Hedgehog, Positive Regulation of Response to DNA Damage Stimulus