LIG3 anticorps (C-Term)

N° du produit ABIN2855575

Détail du produit anti-LIG3 anticorps

Antigène: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIG3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Réactivité croisée: Hamster, Humain

Attributs du produit: Rabbit Polyclonal antibody to DNA ligase III (ligase III, DNA, ATP-dependent)
DNA ligase III antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Classe de qualité: KO Validated

Immunogène: Recombinant protein encompassing a sequence within the C-terminus region of human DNA ligase III. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: 293T

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.15 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-LIG3 (ABIN2855575)

Abdou, Poirier, Hendzel, Weinfeld: "DNA ligase III acts as a DNA strand break sensor in the cellular orchestration of DNA strand break repair." dans: Nucleic acids research, Vol. 43, Issue 2, pp. 875-92, (2015) (PubMed).

Détails sur LIG3

Antigène: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Autre désignation: DNA ligase 3 (LIG3 Produits)

Sujet: This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Cellular Localization: Nucleus

Poids moléculaire: 113 kDa

ID gène: 3980

UniProt: P49916

Pathways: Réparation de l'ADN