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GRP94 anticorps (C-Term)

Name: GRP94 anticorps (C-Term)
Brand: antibodies-online
SKU: ABIN2855586
Price: 630.63 EUR
Availability: InStock

HSP90B1 Reactivité: Humain, Souris, Rat WB, IF, IP, ICC, IHC (p) Hôte: Lapin Polyclonal unconjugated

WB Image Various whole cell extracts (30 μg) were separated by 7. (GRP94 anticorps (C-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded HEP3B xenograft, using GRP94, antibody at 1:100 dilution. (GRP94 anticorps (C-Term))

IHC

ICC/IF Image GRP94 antibody detects GRP94 protein at cytoplasm by immunofluorescent analysis. (GRP94 anticorps (C-Term))

WB Image GRP94 antibody detects GRP94 protein by western blot analysis. (GRP94 anticorps (C-Term))

IP Image Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody, Western blot analysis was performed using GRP94 antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (GRP94 anticorps (C-Term))

WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 7. (GRP94 anticorps (C-Term))

WB Image Sample (30 ug of whole cell lysate) A:NIH-3T3 7. (GRP94 anticorps (C-Term))

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GRP94, antibody (green) at 1:500 dilution. (GRP94 anticorps (C-Term))

8 images

(1 reference)

N° du produit ABIN2855586

Fiche de données

Antigène Voir toutes GRP94 (HSP90B1) Anticorps

GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
Épitope
19

17

16

7

7

7

7

7

6

4

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivité
136

95

60

39

35

31

31

30

28

28

26

26

25

17

10

2

2

1

1
Humain, Souris, Rat
Hôte
99

39

20

2

1
Lapin
Anticorps secondaires appropriés
Clonalité
101

60
Polyclonal
Conjugué
65

11

11

10

9

8

4

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp GRP94 est non-conjugé
Application
141

81

62

60

44

43

34

18

13

13

13

11

4

4

2

2

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Réactivité croisée

Chien, Singe, Porc

Réactivité croisée (Details)

Dog (95 %), Monkey (98 %), Pig (95 %)

Attributs du produit

Rabbit Polyclonal antibody to GRP94 (heat shock protein 90 kDa beta (Grp94), member 1)
GRP94 antibody

Purification

Purified by antigen-affinity chromatography.

Immunogène

Recombinant protein encompassing a sequence within the C-terminus region of human GRP94. The exact sequence is proprietary.

Isotype

IgG

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anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 550-650) antibody
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anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Internal Region) antibody
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anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 676-803) antibody
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anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 16-43), (N-Term) antibody
HSP90B1 Reactivité: Humain, Souris, Hamster WB, FACS, IF, IHC (p) Hôte: Lapin Polyclonal RB25612 unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibody
HSP90B1 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet, Hamster, Chien, Lapin, Porc, Cobaye, Singe, Mouton, Cheval, Xenopus laevis WB, IHC, FACS, IF, StM Hôte: Rat Monoclonal 9G10-F8-2 unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibody
HSP90B1 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet, Hamster, Chien, Lapin, Porc, Cobaye, Singe, Mouton, Cheval, Xenopus laevis IHC, FACS, StM Hôte: Lapin Monoclonal HSP90B1-3168R unconjugated Recombinant Antibody

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibody
HSP90B1 Reactivité: Humain, Souris, Rat WB, IHC, IF, ELISA, ICC Hôte: Lapin Polyclonal unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 687-700), (Middle Region) antibody
HSP90B1 Reactivité: Humain, Souris, Rat WB, ICC, IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 460-487) antibody
HSP90B1 Reactivité: Humain WB, FACS, IHC (p) Hôte: Lapin Polyclonal RB31597 unconjugated

Indications d'application

Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Commentaires

Positive Control: 293T , A431 , HeLa , HepG2 , NIH-3T3 , Mouse brain , PC-12 , Rat-2

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur

Thimerosal (Merthiolate)

Précaution d'utilisation

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C

Stockage commentaire

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Brzozowa-Zasada, Kurek, Piecuch, Wyrobiec: "The clinical and prognostic evaluation of GRP94 immunoexpression in Caucasian patients with colorectal adenocarcinoma." dans: Przeglad gastroenterologiczny, Vol. 14, Issue 2, pp. 140-147, (2019) (PubMed).
Antigène

GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Autre désignation

GRP94 (HSP90B1 Produits)

Synonymes

anticorps ecgp, anticorps gp96, anticorps tra1, anticorps grp94, anticorps DDBDRAFT_0206450, anticorps DDBDRAFT_0215015, anticorps DDB_0206450, anticorps DDB_0215015, anticorps TRA1, anticorps HSP90B1, anticorps ERp99, anticorps GRP94, anticorps TA-3, anticorps Targ2, anticorps Tra-1, anticorps Tra1, anticorps endoplasmin, anticorps GP96, anticorps fb61d09, anticorps tra-1, anticorps wu:fb61d09, anticorps wu:fq25g01, anticorps Grp94, anticorps ECGP, anticorps Gp96, anticorps ppk98, anticorps hsp108, anticorps heat shock protein 90kDa beta (Grp94), member 1, anticorps heat shock protein Hsp90 family protein, anticorps 94 kD glucose-regulated protein, anticorps heat shock protein 90 beta family member 1, anticorps heat shock protein 90, beta (Grp94), member 1, anticorps heat shock protein 90, beta (grp94), member 1, anticorps heat shock protein 90kDa beta family member 1 L homeolog, anticorps hsp90b1, anticorps grp94, anticorps HSP90B1, anticorps Hsp90b1, anticorps hsp90b1.L

Sujet

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1, MIM 140571) and mitochondria (TRAP1, MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Cellular Localization: Endoplasmic reticulum lumen , Melanosome

Poids moléculaire

92 kDa

ID gène

7184

Pathways

Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades