IDH1 anticorps (Center)

N° du produit ABIN2856056

Détail du produit anti-IDH1 anticorps

Antigène: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Épitope: Center

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IDH1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Réactivité croisée: Macaque rhésus

Réactivité croisée (Details): Rhesus Monkey (100 %)

Attributs du produit: Rabbit Polyclonal antibody to IDH1 (isocitrate dehydrogenase 1 (NADP+), soluble)
IDH1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human IDH1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:1000* Western blot1:500-1:3000*

Commentaires:

Positive Control: A431 , HepG2 , JC , C2C12 , 293T , Hela , Neuro2A , GL261 , NIH-3T3 , BCL-1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur IDH1

Antigène: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Autre désignation: IDH1 (IDH1 Produits)

Synonymes: anticorps IDCD, anticorps IDH, anticorps IDP, anticorps IDPC, anticorps PICD, anticorps NADP-CICDH, anticorps AI314845, anticorps AI788952, anticorps E030024J03Rik, anticorps Id-1, anticorps Idh-1, anticorps Idpc, anticorps cb876, anticorps fm90e09, anticorps im:7143416, anticorps wu:fm90e09, anticorps F23E12.180, anticorps F23E12_180, anticorps IDH-I, anticorps NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 1, anticorps isocitrate dehydrogenase 1, anticorps isocitrate dehydrogenase I, anticorps isocitrate dehydrogenase (NADP(+)) 1, cytosolic, anticorps isocitrate dehydrogenase 1 (NADP+), soluble, anticorps isocitrate dehydrogenase 1 (NADP+) L homeolog, anticorps isocitrate dehydrogenase 1, anticorps IDH1, anticorps Idh1, anticorps idh1.L, anticorps idh1

Sujet: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

Cellular Localization: Cytoplasm , Peroxisome

Poids moléculaire: 47 kDa

ID gène: 3417

Pathways: L'effet Warburg