PFKL anticorps (Center)
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- Antigène Voir toutes PFKL Anticorps
- PFKL (Phosphofructokinase, Liver (PFKL))
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Épitope
- Center
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Reactivité
- Humain, Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PFKL est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Réactivité croisée
- Boeuf (Vache), Rat (Rattus)
- Réactivité croisée (Details)
- Rat (95 %), Bovine (94 %)
- Attributs du produit
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Rabbit Polyclonal antibody to PFKL (phosphofructokinase, liver)
PFKL antibody [C1C3] - Purification
- Purified by antigen-affinity chromatography.
- Immunogène
- Recombinant protein encompassing a sequence within the center region of human PFKL. The exact sequence is proprietary.
- Isotype
- IgG
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- Indications d'application
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- Commentaires
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Positive Control: A431 , H1299 , HeLa , HepG2
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.8 mg/mL
- Buffer
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Agent conservateur
- Thimerosal (Merthiolate)
- Précaution d'utilisation
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Antigène
- PFKL (Phosphofructokinase, Liver (PFKL))
- Autre désignation
- PFKL (PFKL Produits)
- Synonymes
- anticorps PFK-B, anticorps AA407869, anticorps phosphofructokinase, liver type, anticorps phosphofructokinase, liver, B-type, anticorps PFKL, anticorps Pfkl
- Sujet
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Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD).
- Poids moléculaire
- 85 kDa
- ID gène
- 5211
- Pathways
- Negative Regulation of Hormone Secretion, L'effet Warburg
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