Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

POLR3A anticorps (N-Term)

POLR3A Reactivité: Humain WB, ICC, IF, IP Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN2856286
  • Antigène Voir toutes POLR3A Anticorps
    POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
    Épitope
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 26
    • 16
    • 11
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 26
    Lapin
    Clonalité
    • 26
    Polyclonal
    Conjugué
    • 15
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp POLR3A est non-conjugé
    Application
    • 14
    • 10
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
     Réactivité croisée
    Souris, Poulet, Xenopus laevis, Boeuf (Vache), Xenopus tropicalis
    Réactivité croisée (Details)
    Mouse (97 %), Chicken (97 %), Xenopus laevis (94 %), Bovine (98 %), Xenopus tropicalis (94 %)
    Attributs du produit
    Rabbit Polyclonal antibody to POLR3A (polymerase (RNA) III (DNA directed) polypeptide A, 155 kDa)
    POLR3A antibody
    Purification
    Purified by antigen-affinity chromatography.
    Immunogène
    Recombinant protein encompassing a sequence within the N-terminus region of human POLR3A. The exact sequence is proprietary.
    Isotype
    IgG
  • Indications d'application
    Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
    Commentaires

    Positive Control: HCT116 , SK-N-SH , IMR32 , SK-N-AS

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.49 mg/mL
    Buffer
    1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Antigène
    POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
    Autre désignation
    POLR3A (POLR3A Produits)
    Synonymes
    anticorps 9330175N20Rik, anticorps BC053071, anticorps RPC1, anticorps RPC155, anticorps RGD1305574, anticorps ADDH, anticorps HLD7, anticorps hRPC155, anticorps polymerase (RNA) III (DNA directed) polypeptide A, anticorps RNA polymerase III subunit A, anticorps Polr3a, anticorps POLR3A
    Sujet
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

    Cellular Localization: Nucleus
    Poids moléculaire
    156 kDa
    ID gène
    11128
Vous êtes ici:
Support technique