RAG2 anticorps (Center)

N° du produit ABIN2856698

Détail du produit anti-RAG2 anticorps

Antigène: RAG2 (Recombination Activating Gene 2 (RAG2))

Épitope: Center

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAG2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Réactivité croisée: Souris, Porc, Lapin, Rat (Rattus), Boeuf (Vache)

Réactivité croisée (Details): Mouse (85 %), Pig (90 %), Rabbit (91 %), Rat (86 %), Bovine (91 %)

Attributs du produit: Rabbit Polyclonal antibody to RAG2 (recombination activating gene 2)
RAG2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human RAG2. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Commentaires:

Positive Control: Molt-4 , rat thymus

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.37 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur RAG2

Antigène: RAG2 (Recombination Activating Gene 2 (RAG2))

Autre désignation: RAG2 (RAG2 Produits)

Synonymes: anticorps RAG-2, anticorps Rag-2, anticorps rag-2, anticorps zgc:136743, anticorps recombination activating 2, anticorps recombination activating gene 2, anticorps recombination activating gene 2 S homeolog, anticorps RAG2, anticorps Rag2, anticorps rag2, anticorps rag2.S

Sujet: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

Cellular Localization: Nucleus

Poids moléculaire: 59 kDa

ID gène: 5897

Pathways: Chromatin Binding, Production of Molecular Mediator of Immune Response