Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Réactivité croisée (Details)
Minimum cross-reactivity to Human, Mouse, and Rat Serum Proteins.1 mg/ml
Purification
Affinity chromatography purified
Stérilité
Filtered
Immunogène
GFP antibody was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.
GFP
Reactivité: Aequorea victoria
WB, IP, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
Sterile filtered liquid in 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, and 0.01 % NaN3.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Stock
4 °C/-20 °C
Stockage commentaire
Store vial at -20 °C prior to opening. Aliquot and freeze at -20 °C or below for long term storage. This product is stable for several weeks at 4 °C as an undiluted liquid.
Buser, Drubin: "Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immunolabeling." dans: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, Vol. 19, Issue 2, pp. 381-92, (2013) (PubMed).
Buser, McDonald: "Correlative GFP-immunoelectron microscopy in yeast." dans: Methods in enzymology, Vol. 470, pp. 603-18, (2010) (PubMed).
anticorps green fluorescent protein, anticorps gfp
Sujet
Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.