HES4 anticorps (AA 94-221)

N° du produit ABIN291868

Détail du produit anti-HES4 anticorps

Antigène: HES4 (Hairy and Enhancer of Split 4 (HES4))

Épitope: AA 94-221

Reactivité: Humain

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp HES4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Specificité: Selected for its ability to recognize human HES-4 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 1 % cross-reactivity with rhHES-1, rhHES-2, rhHES-5, and rhHES-7.

Purification: Affinity purified

Immunogène: E. coli-derived rhHES-4 (aa94-221).

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect HES-4 in HEK293 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 0.2 ng/well.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Détails sur HES4

Antigène: HES4 (Hairy and Enhancer of Split 4 (HES4))

Autre désignation: HES4 (HES4 Produits)

Synonymes: anticorps bHLHb42, anticorps XHairy2a, anticorps Xhairy2, anticorps Xhairy2b, anticorps hairy2, anticorps hairy2a, anticorps hairy2b, anticorps hes1, anticorps hes4a, anticorps hes4b, anticorps Xhairy2a, anticorps hes family bHLH transcription factor 4, anticorps hes family bHLH transcription factor 4 L homeolog, anticorps hes family bHLH transcription factor 4 S homeolog, anticorps HES4, anticorps hes4, anticorps hes4.L, anticorps hes4.S

Sujet: Name/Gene ID: HES4

Synonyms: HES4, BHLHb42, Transcription factor HES-4, BHLH factor Hes4, Hairy and enhancer of split 4, HHES4

ID gène: 57801

UniProt: Q9HCC6