CD1a anticorps (APC)

N° du produit ABIN301984

Détail du produit anti-CD1a anticorps (APC)

Antigène: CD1a

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD1a est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Specificité: The antibody HI149 reacts with an extracellular epitope of CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas. The antibody does not react with peripheral blood T and B lymphocytes, monocytes, granulocytes, platelets and erythrocytes.

Réactivité croisée (Details): Other not tested, Human

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Human thymocytes

Clone: HI149

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD1a (APC) (ABIN301984)

Mayer, Irschick, Moser, Wurm, Huemer, Romani, Irschick: "Characterization of antigen-presenting cells in fresh and cultured human corneas using novel dendritic cell markers." dans: Investigative ophthalmology & visual science, Vol. 48, Issue 10, pp. 4459-67, (2007) (PubMed).

Angel, Lala, Chen, Edgar, Ostrovsky, Dunbar: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." dans: International immunology, Vol. 19, Issue 11, pp. 1271-9, (2007) (PubMed).

Perros, Dorfmüller, Souza, Durand-Gasselin, Mussot, Mazmanian, Hervé, Emilie, Simonneau, Humbert: "Dendritic cell recruitment in lesions of human and experimental pulmonary hypertension." dans: The European respiratory journal, Vol. 29, Issue 3, pp. 462-8, (2007) (PubMed).

Demedts, Brusselle, Vermaelen, Pauwels: "Identification and characterization of human pulmonary dendritic cells." dans: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, (2005) (PubMed).

Chen, Murakami, Oppenheim, Howard: "Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking." dans: Blood, Vol. 106, Issue 7, pp. 2409-16, (2005) (PubMed).

Kirsch, Zeyda, Stuhlmeier, Grisar, Smolen, Watschinger, Stulnig, Hörl, Zlabinger, Säemann: "The active metabolite of leflunomide, A77 1726, interferes with dendritic cell function." dans: Arthritis research & therapy, Vol. 7, Issue 3, pp. R694-703, (2005) (PubMed).

Hunger, Sieling, Ochoa, Sugaya, Burdick, Rea, Brennan, Belisle, Blauvelt, Porcelli, Modlin: "Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells." dans: The Journal of clinical investigation, Vol. 113, Issue 5, pp. 701-8, (2004) (PubMed).

McKenna, Asplund, Kroft: "Immunophenotypic analysis of hematogones (B-lymphocyte precursors) and neoplastic lymphoblasts by 4-color flow cytometry." dans: Leukemia & lymphoma, Vol. 45, Issue 2, pp. 277-85, (2004) (PubMed).

Säemann, Parolini, Böhmig, Kelemen, Krieger, Neumüller, Knarr, Kammlander, Hörl, Diakos, Stuhlmeier, Zlabinger: "Bacterial metabolite interference with maturation of human monocyte-derived dendritic cells." dans: Journal of leukocyte biology, Vol. 71, Issue 2, pp. 238-46, (2002) (PubMed).

Détails sur CD1a

Antigène: CD1a

Autre désignation: CD1a (CD1a Produits)

Synonymes: anticorps CD1, anticorps FCB6, anticorps HTA1, anticorps R4, anticorps T6, anticorps CD1A, anticorps CD1a molecule, anticorps CD1 antigen, anticorps T-cell surface glycoprotein CD1a, anticorps CD1A, anticorps CD1.1, anticorps LOC100156095, anticorps LOC101111409

Sujet: CD1a molecule,CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.,T6, Leu-6, HTA1, FCB6

ID gène: 909

UniProt: P06126

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process