alpha Tubulin anticorps (Biotin)

N° du produit ABIN301997

Détail du produit anti-alpha Tubulin anticorps (Biotin)

Antigène: alpha Tubulin (TUBA1)

Reactivité: Humain, Souris, Porc, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Paramecium, Dindon, Eisenia fetida

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Tubulin est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Specificité: The antibody TU-01 recognizes a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.

Réactivité croisée (Details): Broad species reactivity

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogène: Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.

Clone: TU-01

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.

Commentaires:

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-alpha Tubulin (Biotin) (ABIN301997)

Lukas, Mazna, Valenta, Doubravska, Pospichalova, Vojtechova, Fafilek, Ivanek, Plachy, Novak, Korinek: "Dazap2 modulates transcription driven by the Wnt effector TCF-4." dans: Nucleic acids research, Vol. 37, Issue 9, pp. 3007-20, (2009) (PubMed).

Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." dans: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, (1997) (PubMed).

Smertenko, Blume, Viklický, Opatrný, Dráber: "Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells." dans: Planta, Vol. 201, Issue 3, pp. 349-58, (1997) (PubMed).

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." dans: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, (1996) (PubMed).

Linhartová, Dráber, Dráberová, Viklický: "Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins." dans: The Biochemical journal, Vol. 288 ( Pt 3), pp. 919-24, (1993) (PubMed).

Dráber, Dráberová, Viklický: "Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head." dans: Histochemistry, Vol. 95, Issue 5, pp. 519-24, (1991) (PubMed).

Dráber, Dráberová, Linhartová, Viklický: "Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies." dans: Journal of cell science, Vol. 92 ( Pt 3), pp. 519-28, (1990) (PubMed).

Dráber, Dráberová, Zicconi, Sellitto, Viklický, Cappuccinelli: "Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin." dans: European journal of cell biology, Vol. 41, Issue 1, pp. 82-8, (1987) (PubMed).

Grimm, Breitling, Little: "Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1." dans: Biochimica et biophysica acta, Vol. 914, Issue 1, pp. 83-8, (1987) (PubMed).

Viklický, Dráber, Hasek, Bártek: "Production and characterization of a monoclonal antitubulin antibody." dans: Cell biology international reports, Vol. 6, Issue 8, pp. 725-31, (1982) (PubMed).

Détails sur alpha Tubulin

Antigène: alpha Tubulin (TUBA1)

Autre désignation: alpha-tubulin (TUBA1 Produits)

Synonymes: anticorps Tuba1, anticorps K-ALPHA-1, anticorps Tuba-1, anticorps cb944, anticorps fb22g06, anticorps tuba1, anticorps wu:fb22g06, anticorps Calpha1, anticorps tubulin, alpha 1A, anticorps tubulin alpha 1b, anticorps tubulin, alpha 1b, anticorps tubulin, alpha 1B, anticorps Tuba1a, anticorps TUBA1B, anticorps tuba1b, anticorps Tuba1b

Sujet: Tubulin alpha 1,The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.,TUBA

ID gène: 7277

UniProt: Q71U36

Pathways: Dynamique des Microtubules