CD3 anticorps (PerCP)

N° du produit ABIN302049

Détail du produit anti-CD3 anticorps (PerCP)

Antigène: CD3

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est conjugé à/à la PerCP

Application: Flow Cytometry (FACS)

Specificité: The antibody MEM-57 reacts with an extracellular epitope on gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Human thymocytes and T lymphocytes.

Clone: MEM-57

Isotype: IgG2a kappa

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD3 (PerCP) (ABIN302049)

Majer, Vlaskova, Krol, Kalina, Kubanek, Stolnaya, Dvorakova, Elleder, Sikora: "Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency." dans: Gene, Vol. 498, Issue 2, pp. 183-95, (2012) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." dans: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." dans: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).

Panyi, Bagdány, Bodnár, Vámosi, Szentesi, Jenei, Mátyus, Varga, Waldmann, Gáspar, Damjanovich: "Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2592-7, (2003) (PubMed).

Dave, Cao, Browne, Alarcon, Fernandez-Miguel, Lafaille, de la Hera, Tonegawa, Kappes: "CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage." dans: The EMBO journal, Vol. 16, Issue 6, pp. 1360-70, (1997) (PubMed).

Soucek, Hilgert, Budová, Lindnerová: "Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2." dans: Neoplasma, Vol. 41, Issue 2, pp. 75-81, (1994) (PubMed).

Hilgert, Fran?k, Stefanová, Kaslík, Jirka, Kristofová, Rossmann, Soucek, Horejsi: "Therapeutic in vivo use of the A1-CD3 monoclonal antibody." dans: Transplantation, Vol. 55, Issue 2, pp. 435-8, (1993) (PubMed).

Soucek, Chudomel, Hrubá, Lindnerová: "Induction of NK and LAK activities in human lymphocyte culture by a cytosol fraction from leukemic myeloblasts and by monoclonal antibody CD 3." dans: Neoplasma, Vol. 38, Issue 1, pp. 33-41, (1991) (PubMed).

Détails sur CD3

Antigène: CD3

Autre désignation: CD3 (CD3 Produits)

Synonymes: anticorps CD3, anticorps AI504783, anticorps CD3epsilon, anticorps T3e, anticorps cd3-gamma, anticorps cd3g, anticorps cd3g-a, anticorps cd3g/d, anticorps cd3gd, anticorps t3g, anticorps CD3e molecule, anticorps CD3d molecule, anticorps CD3 antigen, epsilon polypeptide, anticorps CD3g molecule S homeolog, anticorps CD3E, anticorps CD3D, anticorps Cd3e, anticorps cd3g.S

Sujet: CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.,CD3E, T3E, TCRE

ID gène: 916

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway