GST
Reactivité: Différentes espèces
WB, IP, DB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:1000-1:2000, IF: 1: 500, IP: 1:100
Restrictions
For Research Use only
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid freeze / thaw cycles
Stock
-20 °C / -80 °C
Yang, Gu, Cheung, Yan, Tong, Jiang, Yang: "InsP3R-SEC5 interaction on phagosomes modulates innate immunity to Candida albicans by promoting cytosolic Ca2+ elevation and TBK1 activity." dans: BMC biology, Vol. 16, Issue 1, pp. 46, (2018) (PubMed).
Zhao, Tao, Qi, Xu, Yin, Peng: "Protective effect of dioscin against doxorubicin-induced cardiotoxicity via adjusting microRNA-140-5p-mediated myocardial oxidative stress." dans: Redox biology, Vol. 16, pp. 189-198, (2018) (PubMed).
Gou, Kang, Dai, Wang, Li, Zhao, Zhang, Hua, Lu, Zhu, Li, Chen, Wu, Li, Fu, Li, Shi, Liu: "Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis." dans: Cell, Vol. 169, Issue 6, pp. 1090-1104.e13, (2017) (PubMed).
Zhang, Cai, Lin, Tao, Jin: "Subcellular localization and interactions of Infectious Salmon Anemia Virus (ISAV) M1 and NEP as well as host Hsc70." dans: Virology journal, Vol. 14, Issue 1, pp. 30, (2017) (PubMed).
Zhang, Wang, Zhong, Luo, Shang, Liu, Chen, Fang, Xiao: "Ubiquitin-specific Protease 15 Negatively Regulates Virus-induced Type I Interferon Signaling via Catalytically-dependent and -independent Mechanisms." dans: Scientific reports, Vol. 5, pp. 11220, (2016) (PubMed).
Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. Forinstance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase(GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression bothin plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.