TUBG1 anticorps (AA 434-449)

N° du produit ABIN302067

Détail du produit anti-TUBG1 anticorps

Antigène: TUBG1 (Tubulin, gamma 1 (TUBG1))

Épitope: AA 434-449

Reactivité: Plantes, Protozoa

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TUBG1 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Specificité: The antibody TU-32 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.

Réactivité croisée (Details): Animals, Protozoa, Plants

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449

Clone: TU-32

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.
Immunocytochemistry: Methanol/acetone fixation required.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-TUBG1 (ABIN302067)

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." dans: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, (2007) (PubMed).

Katsetos, Reddy, Dráberová, Smejkalová, Del Valle, Ashraf, Tadevosyan, Yelin, Maraziotis, Mishra, Mörk, Legido, Nissanov, Baas, de Chadarévian, Dráber: "Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines." dans: Journal of neuropathology and experimental neurology, Vol. 65, Issue 5, pp. 465-77, (2006) (PubMed).

Sulimenko, Dráberová, Sulimenko, Macurek, Richterová, Dráber, Dráber: "Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 12, pp. 7243-53, (2006) (PubMed).

Libusová, Sulimenko, Sulimenko, Hozák, Dráber: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." dans: Experimental cell research, Vol. 295, Issue 2, pp. 375-86, (2004) (PubMed).

Linhartová, Novotná, Sulimenko, Dráberová, Dráber: "Gamma-tubulin in chicken erythrocytes: changes in localization during cell differentiation and characterization of cytoplasmic complexes." dans: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 223, Issue 2, pp. 229-40, (2002) (PubMed).

Sulimenko, Sulimenko, Poznanovic, Nechiporuk-Zloy, Böhm, Macurek, Unger, Dráber: "Association of brain gamma-tubulins with alpha beta-tubulin dimers." dans: The Biochemical journal, Vol. 365, Issue Pt 3, pp. 889-95, (2002) (PubMed).

Dráberová, Mac?rek, Richterová, Böhm, Dráber: "Monoclonal antibody KN-01 against the heavy chain of kinesin." dans: Folia biologica, Vol. 48, Issue 2, pp. 77-9, (2002) (PubMed).

Nohýnkova, Dráber, Reischig, Kulda: "Localization of gamma-tubulin in interphase and mitotic cells of a unicellular eukaryote, Giardia intestinalis." dans: European journal of cell biology, Vol. 79, Issue 6, pp. 438-45, (2000) (PubMed).

Binarová, Cenklová, Hause, Kubátová, Lysák, Dolezel, Bögre, Dráber: "Nuclear gamma-tubulin during acentriolar plant mitosis." dans: The Plant cell, Vol. 12, Issue 3, pp. 433-42, (2000) (PubMed).

Dráberová, Dráberová, Surviladze, Dráber, Dráber: "Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells." dans: International immunology, Vol. 11, Issue 11, pp. 1829-39, (1999) (PubMed).

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." dans: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, (1996) (PubMed).

Détails sur TUBG1

Antigène: TUBG1 (Tubulin, gamma 1 (TUBG1))

Autre désignation: gamma-tubulin (TUBG1 Produits)

Synonymes: anticorps GCP-1, anticorps TUBG, anticorps TUBGCP1, anticorps TUBC, anticorps TUBG3, anticorps gamma-tubulin, anticorps Tubg2, anticorps xgam, anticorps 1500010O08Rik, anticorps AI451582, anticorps AI503389, anticorps Tubg, anticorps tubgl, anticorps zgc:55894, anticorps tubulin gamma 1, anticorps gamma-tubulin 1, anticorps gamma-tubulin, anticorps tubulin gamma 1 L homeolog, anticorps Tubulin gamma chain, anticorps tubulin, gamma 1, anticorps tubulin gamma-1 chain, anticorps TUBG1, anticorps tubg1, anticorps tubg1.L, anticorps tbg-1, anticorps Tubg1, anticorps LOC101686824, anticorps LOC101103906

Sujet: Tubulin gamma 1,The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. Humans possess two gamma-tubulin genes. Gamma-tubulin 1 represents a ubiquitous isotype, whereas gamma-tubulin 2 is found predominantly in the brain, where it may be endowed with divergent functions beyond microtubule nucleation.,TUBG

ID gène: 7283

UniProt: P23258

Pathways: Dynamique des Microtubules, M Phase