The antibody MEM-E/08 recognizes an extracellular epitope on native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells. The published results revealed that antibody is remarkably specific for HLA-E, only with weak cross-reactivity with following classical MHC Class I molecules (MHC Class Ia): HLA-A24, -B7, -B27, -B51, -B54, -C7.
Réactivité croisée (Details)
Human
Purification
Purified by protein-A affinity chromatography.
Pureté
> 95 % (by SDS-PAGE)
Immunogène
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Allard, Oger, Vignard, Percier, Fregni, Périer, Caignard, Charreau, Bernardeau, Khammari, Dréno, Gervois: "Serum soluble HLA-E in melanoma: a new potential immune-related marker in cancer." dans: PLoS ONE, Vol. 6, Issue 6, pp. e21118, (2011) (PubMed).
Lo Monaco, Tremante, Cerboni, Melucci, Sibilio, Zingoni, Nicotra, Natali, Giacomini: "Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells." dans: Neoplasia (New York, N.Y.), Vol. 13, Issue 9, pp. 822-30, (2011) (PubMed).
Gonen-Gross, Achdout, Arnon, Gazit, Stern, Horejsí, Goldman-Wohl, Yagel, Mandelboim: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, (2005) (PubMed).
Palmisano, Contardi, Morabito, Gargaglione, Ferrara, Pistillo: "HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines." dans: Human immunology, Vol. 66, Issue 1, pp. 1-12, (2004) (PubMed).
Antigène
HLA-E
(HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))
anticorps EA1.2, anticorps EA2.1, anticorps HLA-6.2, anticorps MHC, anticorps QA1, anticorps major histocompatibility complex, class I, E, anticorps HLA-E, anticorps PATR-E
Sujet
Major histocompatibility complex, class I, E,HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).,HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E