Myc Tag anticorps (C-Term)

N° du produit ABIN302092

Détail du produit anti-Myc Tag anticorps

Antigène: Myc Tag

Épitope: C-Term

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Myc Tag est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Specificité: The antibody 9E10 can be used to detect the c-Myc tag.

Réactivité croisée (Details): Human, Recognizes fusion proteins in all species

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.

Clone: 9E10

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/mL, positive tissue: perfused brain sections, liver, spleen.
Immunoprecipitation: Recommended dilution: 1-5 μg/mL, this antibody is not suitable for immunoprecipitation of native c-Myc protein.
Flow cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-4 μg/mL.
Western blotting: Recommended dilution: 0,5-2 μg/mL, positive control: c-Myc tagged protein.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-Myc Tag (ABIN302092)

Jelínková, Šafaříková, Vondálová Blanářová, Skender, Hofmanová, Sova, Moyer, Kozubík, Kolář, Ehrmann, Hyršlová Vaculová: "Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway." dans: Biochemical pharmacology, Vol. 92, Issue 3, pp. 415-24, (2014) (PubMed).

Veracini, Simon, Richard, Schraven, Horejsi, Roche, Benistant: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." dans: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, (2008) (PubMed).

Wang, Campoli, Ko, Luo, Ferrone: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." dans: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, (2004) (PubMed).

Fujiwara, Poikonen, Aleman, Valtavaara, Saksela, Mayer: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." dans: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, (2002) (PubMed).

Baggio, Burgstaller, Hale, Putney, Lane, Lipovsek, Wright, Roberts, Liu, Szostak, Wagner: "Identification of epitope-like consensus motifs using mRNA display." dans: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, (2002) (PubMed).

Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." dans: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).

Hilpert, Hansen, Wessner, Küttner, Welfle, Seifert, Höhne: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." dans: Protein engineering, Vol. 14, Issue 10, pp. 803-6, (2001) (PubMed).

Siegel, Brandner, Hess: "Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC." dans: International journal of oncology, Vol. 13, Issue 6, pp. 1259-62, (1999) (PubMed).

Détails sur Myc Tag

Antigène: Myc Tag

Autre désignation: c-Myc tag (Myc Tag Produits)

Classe de substances: Tag

Sujet: MYC proto-oncogene,The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.,bHLH, MRTL, MYCC

ID gène: 4609

UniProt: P01106