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FZD2 anticorps (Internal Region)

Verified FZD2 Reactivité: Humain WB, ELISA, IF, IHC Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN302136
  • Antigène Voir toutes FZD2 Anticorps
    FZD2 (Frizzled Family Receptor 2 (FZD2))
    Épitope
    • 8
    • 8
    • 5
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivité
    • 39
    • 19
    • 16
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    Humain
    Hôte
    • 38
    • 2
    • 1
    • 1
    Chèvre
    Clonalité
    • 39
    • 3
    Polyclonal
    Conjugué
    • 29
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp FZD2 est non-conjugé
    Application
    • 28
    • 17
    • 6
    • 6
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
    Fonction
    Frizzled 2
    Séquence
    RYATLEHPFH CPR
     Réactivité croisée
    Humain, Souris, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.
    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 60-65 kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24 kDa was also observed in some lysates which was successfully blocked by incubation with

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Hirano, Yonezawa, Yunoue, Habu, Uchida, Yoshioka, Kishida, Kishida, Oyoshi, Fujio, Sugata, Yamahata, Hanaya, Arita: "Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma: evaluative methodology for DAB chromogenic immunostaining." dans: Brain tumor pathology, Vol. 31, Issue 2, pp. 85-93, (2014) (PubMed).

  • Antigène
    FZD2 (Frizzled Family Receptor 2 (FZD2))
    Autre désignation
    FZD2 (FZD2 Produits)
    Sujet
    FZD2 , frizzled homolog 2 (Drosophila) , frizzled 2
    ID gène
    2535, 57265, 64512
    NCBI Accession
    NP_001457
    Pathways
    Signalisation WNT
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