FZD2 anticorps (Internal Region)
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- Antigène Voir toutes FZD2 Anticorps
- FZD2 (Frizzled Family Receptor 2 (FZD2))
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Épitope
- Internal Region
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Reactivité
- Humain
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp FZD2 est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
- Fonction
- Frizzled 2
- Séquence
- RYATLEHPFH CPR
- Réactivité croisée
- Humain, Souris, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Classe de qualité
- Verified
- Immunogène
- Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.
- Isotype
- IgG
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- Indications d'application
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Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 60-65 kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24 kDa was also observed in some lysates which was successfully blocked by incubation with
Peptide ELISA: antibody detection limit dilution 1:64000.
- Commentaires
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Minimize freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma: evaluative methodology for DAB chromogenic immunostaining." dans: Brain tumor pathology, Vol. 31, Issue 2, pp. 85-93, (2014) (PubMed).
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Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma: evaluative methodology for DAB chromogenic immunostaining." dans: Brain tumor pathology, Vol. 31, Issue 2, pp. 85-93, (2014) (PubMed).
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- Antigène
- FZD2 (Frizzled Family Receptor 2 (FZD2))
- Autre désignation
- FZD2 (FZD2 Produits)
- Sujet
- FZD2 , frizzled homolog 2 (Drosophila) , frizzled 2
- ID gène
- 2535, 57265, 64512
- NCBI Accession
- NP_001457
- Pathways
- Signalisation WNT
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