Complement Factor H anticorps (AA 20-270)

N° du produit ABIN3023097

Détail du produit anti-Complement Factor H anticorps

Antigène: Complement Factor H (CFH)

Épitope: AA 20-270

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Complement Factor H est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Séquence: DCNELPPRRN TEILTGSWSD QTYPEGTQAI YKCRPGYRSL GNVIMVCRKG EWVALNPLRK CQKRPCGHPG DTPFGTFTLT GGNVFEYGVK AVYTCNEGYQ LLGEINYREC DTDGWTNDIP ICEVVKCLPV TAPENGKIVS SAMEPDREYH FGQAVRFVCN SGYKIEGDEE MHCSDDGFWS KEKPKCVEIS CKSPDVINGS PISQKIIYKE NERFQYKCNM GYEYSERGDA VCTESGWRPL PSCEEKSCDN P

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Polyclonal Antibodies

Purification: Affinity purification

Immunogène: Recombinant fusion protein containing a sequence corresponding to amino acids 20-270 of human Complement factor H (NP_001014975.1).

Isotype: IgG

Information d'application

Indications d'application: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Independent Validation (IF)

Validation #101403 (Immunofluorescence)

by: Alexander Lab, Jacobs School of Medicine and Biomedical Sciences, Department of Medicine, University of Buffalo

No.: #101403

Date: 19.07.2017

Antigène: Commplement Factor H

Numéro du lot: 4390101

Application validée: Immunofluorescence

Contrôle positif: MRL/lpr lupus mice (14 weeks of age)

Contrôle négative: FH knockout MRL/lpr lupus mice

Conclusion: Passed. The complement factor H antibody ABIN3023097 works very well on murine kidney samples and shows the expected CFH localization and strong staining.

Validation Images

IF staining with ABIN3023097 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.

Protocole

Anticorps primaire: ABIN3023097

Anticorps secondaire: goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)

Full Protocol:

• Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
• Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
• Sections were cut at 8µm using the cryostat.
• Dry sections for 30min at RT.
• Hydrate sections in PBS 2x for 15min at RT.
• Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
• Wash sections 2x with PBS for 15min at RT.
• Incubate sections with primary rabbit anti-Complement Factor H antibody (antibodies-online, ABIN3023097, 0004390101) diluted 1:100 in PBS ON at 4°C.
• Wash sections 2x for 15min with PBS.
• Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze / thaw cycles

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze / thaw cycles.

Références pour anticorps anti-Complement Factor H (ABIN3023097)

Cheng, Malachi, Cipi, Ma, Brush, Agbaga, Punzo: "HK2 Mediated Glycolytic Metabolism in Mouse Photoreceptors Is Not Required to Cause Late Stage Age-Related Macular Degeneration-Like Pathologies." dans: Biomolecules, Vol. 11, Issue 6, (2021) (PubMed).

Détails sur Complement Factor H

Antigène: Complement Factor H (CFH)

Autre désignation: CFH (CFH Produits)

Synonymes: anticorps AHUS1, anticorps AMBP1, anticorps ARMD4, anticorps ARMS1, anticorps CFHL3, anticorps FH, anticorps FHL1, anticorps HF, anticorps HF1, anticorps HF2, anticorps HUS, anticorps Mud-1, anticorps NOM, anticorps Sas-1, anticorps Sas1, anticorps Fh, anticorps CFH, anticorps im:7147412, anticorps si:ch211-207o17.2, anticorps fH, anticorps armd4, anticorps arms1, anticorps cfh, anticorps cfhl3, anticorps fhl1, anticorps hf1, anticorps hf2, anticorps hus, anticorps complement factor H, anticorps complement component factor h, anticorps complement factor H-related protein 5, anticorps complement factor H L homeolog, anticorps CFH, anticorps Cfh, anticorps cfh, anticorps LOC100399888, anticorps LOC100733902, anticorps cfh.L

Sujet: This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.,CFH,AHUS1,AMBP1,ARMD4,ARMS1,CFHL3,FH,FHL1,HF,HF1,HF2,HUS,Immunology & Inflammation,Cell Intrinsic Innate Immunity Signaling Pathway,CFH

Poids moléculaire: 51 kDa/139 kDa

ID gène: 3075

UniProt: P08603

Pathways: Système du Complément, Cellular Response to Molecule of Bacterial Origin