mAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.
Purification
Protein G affinity chromatography
Immunogène
The human hepatocellular carcinoma cell line SK-H1A9-2 was used as the immunogen for this Hepatocyte Specific Antigen antibody.
The optimal dilution of the Hepatocyte Specific Antigen antibody for each application should be determined by the researcher.\. Immunofluorescence: 0.5-1 μg/mL,Immunocytochemistry (Acetone-fixed cells): 0.5-1 μg/mL for 30 minutes at RT
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
1 mg/mL in 1X PBS, BSA free, sodium azide free
Agent conservateur
Azide free
Stock
4 °C,-20 °C
Stockage commentaire
Store the Hepatocyte Specific Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
MAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.