Nuclear Antigen anticorps
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- Antigène
- Nuclear Antigen
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Reactivité
- Humain, Rat, Souris
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Inconjugué
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Application
- Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Attributs du produit
- This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
- Purification
- Protein G affinity chromatography
- Immunogène
- Human cell nuclei were used as the immunogen for the Nuclear Antigen antibody.
- Clone
- NUCM1-1
- Isotype
- IgG1 kappa
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- Indications d'application
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Titering of the Nuclear Antigen antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH 6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunocytochemistry (Acetone-fixed): 0.25-0.5 μg/mL for 30 min at RT,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Agent conservateur
- Azide free
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the Nuclear Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Antigène
- Nuclear Antigen
- Sujet
- This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
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