TOP1MT anticorps
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- Antigène Voir toutes TOP1MT Anticorps
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp TOP1MT est non-conjugé
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogène
- Recombinant full-length human protein was used as the immunogen for the TOP1MT antibody.
- Clone
- TOP1MT-488
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product TOP1MT Anticorps primaire
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- Indications d'application
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Optimal dilution of the TOP1MT antibody should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the TOP1MT antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Antigène
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
- Autre désignation
- TOP1MT (TOP1MT Produits)
- Synonymes
- anticorps 2900052H09Rik, anticorps TOP1, anticorps topoisomerase (DNA) I, mitochondrial, anticorps DNA topoisomerase 1, mitochondrial, anticorps DNA topoisomerase I mitochondrial, anticorps TOP1MT, anticorps Top1mt
- Sujet
- DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
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