MUC1 anticorps

N° du produit ABIN3025281

Détail du produit anti-MUC1 anticorps

Antigène: MUC1 (Mucin 1 (MUC1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MUC1 est non-conjugé

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Pureté: Protein G affinity chromatography

Immunogène: Human milk fat globule membranes were used as the immunogen for this MUC-1 antibody.

Clone: MUC1-955

Isotype: IgG1 kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC-1 antibody to be titered up or down for optimal performance.

1. FFPE staining REQUIRES boiling sections in 10  mM citrate buffer,  pH 6, for 10-20 min followed by cooling at RT for 10-20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: order BSA free format,FACS: 0.5-1 μg/million cells,Immunofluorescence: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the MUC-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur MUC1

Antigène: MUC1 (Mucin 1 (MUC1))

Autre désignation: MUC-1 (MUC1 Produits)

Synonymes: anticorps CA 15-3, anticorps CD227, anticorps EMA, anticorps H23AG, anticorps KL-6, anticorps MAM6, anticorps MCKD1, anticorps MUC-1, anticorps MUC-1/SEC, anticorps MUC-1/X, anticorps MUC1/ZD, anticorps PEM, anticorps PEMT, anticorps PUM, anticorps Muc-1, anticorps mucin 1, cell surface associated, anticorps mucin 1, transmembrane, anticorps MUC1, anticorps Muc1

Sujet: Mucin-1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed in >90 % breast carcinomas and metastases. The transgenic protein has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of the protein uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Mucin-1 antibody is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. The specific epitope of this Mucin-1 antibody has not yet been determined.

ID gène: 4582

Pathways: Negative Regulation of intrinsic apoptotic Signaling