Calponin anticorps

N° du produit ABIN3025295

Détail du produit anti-Calponin anticorps

Antigène: Calponin

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Calponin est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for the anti-Calponin antibody.

Clone: CNN1-832

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the anti-Calponin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the anti-Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Calponin

Antigène: Calponin

Abstract: Calponin Produits

Synonymes: anticorps CalPoNin, anticorps cpn-1, anticorps cpn-2

Sujet: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34  kDa form and a 29  kDa form. Expression of the 29  kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.