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HLA-DP/DR anticorps

HLA-DP/DR Reactivité: Humain FACS, IHC (p), IF Hôte: Souris Monoclonal Bra-14 unconjugated
N° du produit ABIN3025464
  • Antigène Tous les produits HLA-DP/DR
    HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))
    Reactivité
    • 16
    • 5
    Humain
    Hôte
    • 16
    Souris
    Clonalité
    • 16
    Monoclonal
    Conjugué
    • 4
    • 4
    • 4
    • 2
    • 2
    Cet anticorp HLA-DP/DR est non-conjugé
    Application
    • 16
    • 8
    • 8
    • 4
    • 2
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Attributs du produit
    Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
    Purification
    Protein G affinity chromatography
    Immunogène
    Human REH cells were used as the immunogen for the HLA-DR antibody (HLA-DP).
    Clone
    Bra-14
    Isotype
    IgG3 kappa
  • Indications d'application
    Optimal dilution of the HLA-DR antibody (HLA-DP) should be determined by the researcher.

    1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store the HLA-DR antibody (HLA-DP) at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Antigène
    HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))
    Autre désignation
    HLA-DR (HLA-DP) (HLA-DP/DR Produits)
    Sujet
    Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36  kDa) and a chain (27  kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
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