Nucleolin anticorps
-
- Antigène Voir toutes Nucleolin (NCL) Anticorps
- Nucleolin (NCL)
-
Reactivité
- Humain, Souris, Rat, Boeuf (Vache), Porc, Lapin, Cobaye, Poulet, Cat, Gerbille
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp Nucleolin est non-conjugé
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogène
- Recombinant human protein was used as the immunogen for the Nucleolin antibody.
- Clone
- NCL-902
- Isotype
- IgG1
- Top Product
- Discover our top product NCL Anticorps primaire
-
-
- Indications d'application
-
Optimal dilution of the Nucleolin antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,IF: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Western blot: 0.5-1 μg/mL,Prediluted format: incubate for 30 min at RT (2) - Restrictions
- For Research Use only
-
- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Agent conservateur
- Azide free
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
- Antigène
- Nucleolin (NCL)
- Autre désignation
- Nucleolin (NCL Produits)
- Synonymes
- anticorps C23, anticorps B530004O11Rik, anticorps D0Nds28, anticorps D1Nds28, anticorps Nucl, anticorps wu:fa12d03, anticorps zgc:152810, anticorps nucl, anticorps LOC100282923, anticorps LOC100283058, anticorps nucleolin, anticorps nucleolin S homeolog, anticorps NCL, anticorps Ncl, anticorps ncl.S, anticorps ncl, anticorps LOC100195537, anticorps LOC100282923, anticorps LOC100283058
- Sujet
- Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
- Pathways
- Ribonucleoprotein Complex Subunit Organization
-