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Nuclear Antigen anticorps

IPO-38 Reactivité: Humain, Souris, Rat FACS, IF, IHC (p) Hôte: Souris Monoclonal IPO-38 unconjugated
N° du produit ABIN3025764
  • Antigène Voir toutes Nuclear Antigen (IPO-38) Anticorps
    Nuclear Antigen (IPO-38)
    Reactivité
    Humain, Souris, Rat
    Hôte
    • 5
    Souris
    Clonalité
    • 5
    Monoclonal
    Conjugué
    • 5
    Cet anticorp Nuclear Antigen est non-conjugé
    Application
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    PEG precipitation
    Immunogène
    Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO-38 antibody.
    Clone
    IPO-38
    Isotype
    IgM kappa
    Top Product
    Discover our top product IPO-38 Anticorps primaire
  • Indications d'application
    Optimal dilution of the IPO-38 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Agent conservateur
    Azide free
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store the IPO-38 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Antigène
    Nuclear Antigen (IPO-38)
    Autre désignation
    IPO-38 (IPO-38 Produits)
    Sujet
    Recognizes a protein of 14-16  kDa, which is a novel nuclear antigen of proliferating cells. IPO38 antigen is present in the nuclei of proliferating cells such as Hodgkin s disease and non-Hodgkin s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO38 may be a useful marker of cell proliferation during monitoring of tumor progression.
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