CD86 anticorps
-
- Antigène Voir toutes CD86 Anticorps
- CD86
-
Reactivité
- Humain, Souris, Rat
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp CD86 est non-conjugé
-
Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogène
- Recombinant human full-length protein was used as the immunogen for the CD86 antibody.
- Clone
- C86-1146
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD86 Anticorps primaire
-
-
- Indications d'application
-
Optimal dilution of the CD86 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)
- Restrictions
- For Research Use only
-
- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the CD86 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
- Antigène
- CD86
- Autre désignation
- CD86 (CD86 Produits)
- Synonymes
- anticorps B7-2, anticorps B7.2, anticorps B70, anticorps CD28LG2, anticorps LAB72, anticorps B7, anticorps CLS1, anticorps Cd28l2, anticorps ETC-1, anticorps Ly-58, anticorps Ly58, anticorps MB7, anticorps MB7-2, anticorps TS/A-2, anticorps CD86 molecule, anticorps CD86 antigen, anticorps integrin subunit alpha 2b, anticorps CD86, anticorps Cd86, anticorps Itga2b
- Sujet
- Recognizes a protein of 70 kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
-