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Secretory Component Glycoprotein anticorps

Reactivité: Humain, Rat IHC (p), IF, FACS Hôte: Souris Monoclonal SPM217 unconjugated
N° du produit ABIN3026937
  • Antigène Tous les produits Secretory Component Glycoprotein
    Secretory Component Glycoprotein
    Reactivité
    Humain, Rat
    Hôte
    • 3
    Souris
    Clonalité
    • 3
    Monoclonal
    Conjugué
    • 3
    Cet anticorp Secretory Component Glycoprotein est non-conjugé
    Application
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)
    Attributs du produit
    This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
    Purification
    Protein G affinity chromatography
    Immunogène
    Secretory Component protein isolated from human colostrum was used as the immunogen for this Secretory Component antibody.
    Clone
    SPM217
    Isotype
    IgG1 kappa
  • Indications d'application
    The optimal dilution of the Secretory Component antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Agent conservateur
    Azide free
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store the Secretory Component antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Antigène
    Secretory Component Glycoprotein
    Abstract
    Secretory Component Glycoprotein Produits
    Sujet
    This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
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